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《分子生物學：從基因到蛋白質（第3版）（影印版）》前兩版書名是《分子生物學》，分別在1983年和1987年出版，由先後在Brandeis大學和加州大學聖地亞哥分校教授生物化學和分子生物學課程的DavidFreifelder教授編寫并修訂的。
目次
Preface
CHAPTER 1 Introduction to Molecular Biology
SECTION 1 Protein Structure and Function
CHAPTER 2 Protein Structure
CHAPTER 3 Protein Function
SECTION 2 Nucleic Acids and Nucleoproteins
CHAPTER 4 Deoxyribonucleic Acid Structure
CHAPTER 5 Nucleic Acid Technology
CHAPTER 6 Chromosome Structure
SECTION 3 Genetics and Virology
CHAPTER 7 Genetic Analysis in Molecular Biology
CHAPTER 8 Viruses in Molecular Biology
SECTION 4 DNA Metabolism
CHAPTER 9 DNA Replication
CHAPTER 10 DNA Damage and Repair
CHAPTER 11 Recombination
CHAPTER 12 Transposons and Other Mobile Elements
SECTION 5 RNA Synthesis and Processing
CHAPTER 13 Bacterial RNA Polymerase
CHAPTER 14 Regulation of Bacterial Gene Transcription
CHAPTER 15 RNA Polymerase Ⅱ: Basal Transcription
CHAPTER 16 RNA Polymerase Ⅱ: Regulation
CHAPTER 17 RNA Polymerase Ⅱ: Cotranscriptional and Posttranscriptional Processes
CHAPTER 18 Ribosomal RNA, Transfer RNA, and Organellar RNA Synthesis
SECTION 6 Protein Synthesis
CHAPTER 19 Protein Synthesis: The Genetic Code
CHAPTER 20 Protein Synthesis: The Ribosome
Index
CHAPTER 1 Introduction to Molecular Biology
SECTION 1 Protein Structure and Function
CHAPTER 2 Protein Structure
CHAPTER 3 Protein Function
SECTION 2 Nucleic Acids and Nucleoproteins
CHAPTER 4 Deoxyribonucleic Acid Structure
CHAPTER 5 Nucleic Acid Technology
CHAPTER 6 Chromosome Structure
SECTION 3 Genetics and Virology
CHAPTER 7 Genetic Analysis in Molecular Biology
CHAPTER 8 Viruses in Molecular Biology
SECTION 4 DNA Metabolism
CHAPTER 9 DNA Replication
CHAPTER 10 DNA Damage and Repair
CHAPTER 11 Recombination
CHAPTER 12 Transposons and Other Mobile Elements
SECTION 5 RNA Synthesis and Processing
CHAPTER 13 Bacterial RNA Polymerase
CHAPTER 14 Regulation of Bacterial Gene Transcription
CHAPTER 15 RNA Polymerase Ⅱ: Basal Transcription
CHAPTER 16 RNA Polymerase Ⅱ: Regulation
CHAPTER 17 RNA Polymerase Ⅱ: Cotranscriptional and Posttranscriptional Processes
CHAPTER 18 Ribosomal RNA, Transfer RNA, and Organellar RNA Synthesis
SECTION 6 Protein Synthesis
CHAPTER 19 Protein Synthesis: The Genetic Code
CHAPTER 20 Protein Synthesis: The Ribosome
Index
書摘/試閱
These nucleases, which were called restriction endonudeasesbecause they blocked or restricted viral replication, act only on DNAwith specific recognition sequences and only when the recognition sequences are not modified. Host DNA is protected because it has methylgroups attached to specific bases within the recognition sequence.
Three major types of restrictionmodification systems have beenstudied （Table S.2）. Type I restrictionmodification systems consist offive polypeptide subunits: two identical restriction endonuclease subunits （R）, two identical modification subunits （M）, and a specificitysubunit （S）. If the sequence that is recognized by the specificity subunitdoes not have a methyl group, then one of two things will happen. Themodification subunits will methylate the sequence and the DNA willbe protected, or the restriction subunits will cleave the DNA at a nonspecific site, often I kb or more from the recognition sequence, and theDNA will be degraded. Type II restrictionmodification systems aremade of two independent enzymes, a homodimeric restriction endonuclease and a monomeric methyl transferase （methylase）. Type 1I restrictionmodification enzymes recognize sequences that are 4 to 8 bp long.Type II methylases transfer methyl groups to bases within the recognition sequence and type II endonucleases cleave DNA within the recognition sequence. Type III restrictionmodification systems consist oftwo subunits, a modification subunit and a restriction subunit. Modification occurs within the recognition sequence but cleavage takesplace about 25 bp away from this site. The discussion that follows islimited to the type II endonucleases because they are the only one of thethree types that has been widely used to manipulate DNA.
Three major types of restrictionmodification systems have beenstudied （Table S.2）. Type I restrictionmodification systems consist offive polypeptide subunits: two identical restriction endonuclease subunits （R）, two identical modification subunits （M）, and a specificitysubunit （S）. If the sequence that is recognized by the specificity subunitdoes not have a methyl group, then one of two things will happen. Themodification subunits will methylate the sequence and the DNA willbe protected, or the restriction subunits will cleave the DNA at a nonspecific site, often I kb or more from the recognition sequence, and theDNA will be degraded. Type II restrictionmodification systems aremade of two independent enzymes, a homodimeric restriction endonuclease and a monomeric methyl transferase （methylase）. Type 1I restrictionmodification enzymes recognize sequences that are 4 to 8 bp long.Type II methylases transfer methyl groups to bases within the recognition sequence and type II endonucleases cleave DNA within the recognition sequence. Type III restrictionmodification systems consist oftwo subunits, a modification subunit and a restriction subunit. Modification occurs within the recognition sequence but cleavage takesplace about 25 bp away from this site. The discussion that follows islimited to the type II endonucleases because they are the only one of thethree types that has been widely used to manipulate DNA.
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