Gene Regulation—Methods and Protocols

In this volume of Methods in Molecular Biology?, expert investigators offer comprehensive, complementary, and cutting-edge technologies for studies of gene regulation. The chapters of Gene Regulation: Methods and Protocols are organized to provide an integrated and a coherent view of control systems and their associated components. The protocols are broad in their scope. They include molecular, biochemical, spectroscopic techniques as well as high throughput strategies. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines including molecular biology, genomics, biochemistry, biomedicine, nutrition, and agricultural sciences. FEATURES· Techniques for studying chromatin structural features that influence gene activation· In vivo and in vitro assays· Spectroscopic techniques· Technologies for examining genome-wide association of transcription factors with regulatory regions of genes· Proteomics based on SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture) Peptide microarray technology for studies of kinases· Schemes for isolation and characterization of relatively large multiprotein complexes· Biochemical and biophysical techniques for examining gene repression· In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes· Nuclear recruitment assay to validate transcription factor interactions in mammalian cells· Schemes for isolation and characterization of relatively large multiprotein complexes· Biochemical and biophysical techniques for examining gene repression· In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes· Nuclear recruitment assay to validate transcription factor interactions in mammalian cells· Schemes for isolation and characterization of relatively large multiprotein complexes· Biochemical and biophysical techniques for examining gene repression· In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes· Nuclear recruitment assay to validate transcription factor interactions in mammalian cells FEATURES· Techniques for studying chromatin structural features that influence gene activation· In vivo and in vitro assays· Spectroscopic techniques· Technologies for examining genome-wide association of transcription factors with regulatory regions of genes· Proteomics based on SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture) Peptide microarray technology for studies of kinases· Schemes for isolation and characterization of relatively large multiprotein complexes· Biochemical and biophysical techniques for examining gene repression· In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes· Nuclear recruitment assay to validate transcription factor interactions in mammalian cells· Schemes for isolation and characterization of relatively large multiprotein complexes· Biochemical and biophysical techniques for examining gene repression· In vitro chromatin reconstitution to investigate multicomponent transcriptional complexes· Nuclear recruitment assay to validate transcription factor interactions in mammalian cells· Spectroscopic techniques· Technologies for examining genome-wide association of transcription factors with regulatory regions of genes· Proteomics based on SILAC (Stable Isotope Labeling of Amino Acids in Cell Culture) Peptide microarra