Series Preface...
Preface...
Table of Contents...
Contributing Authors...
Part I Molecular Techniques
1. Production of High-Quality Antibodies for the Study of Receptors and Ion Channels
Masahiko Watanabe
2. Co-Immunoprecipitation from Brain
Xavier Morat? Dasiel O. Borroto-Escuela, Kjell Fuxe, V獳tor Fern嫕dez-Due鎙s, and Francisco Ciruela
3. Sub-Synaptic Membrane Fractionation
Paula M. Canas and Rodrigo A. Cunha
4. Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation
Elek Moln嫫
5. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells
Juan Varela, Julien Dupuis, Fran蔞is Maingret, and Laurent Groc
6. Radioligand Binding Detection of Receptors in Brain Membranes
Fuencisla Pilar-Cu幨lar, Emilio Garro-Mart璯ez, Elena Castro, and 翼varo D燰z
7. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain
Markus U. Ehrengruber and Kenneth Lundstrom
8. Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain
V獳tor Fern嫕dez-Due鎙s, Thierry Durroux, and Francisco Ciruela
9. Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay
Manuel Narv墈z, Minerva Crespo-Ram甏ez, Ram鏮 Fores-Pons, Mariana Pita-Rodr璲uez, Francisco Ciruela, Malgorzata Filip, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Miguel Perezde la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela
10. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain
Marta Valle-Le鏮, V獳tor Fern嫕dez-Due鎙s, and Francisco Ciruela
Part II Neuroanatomical Techniques
11. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling
Miwako Yamasaki and Masahiko Watanabe
12. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain
Rebeca Vidal, Fuencisla Pilar-Cuellar, 翼varo D燰z, and Elena Castro
13. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting
Elek Moln嫫
14. Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility
Kohtarou Konno and Masahiko Watanabe
15. Localization of GFP-Tagged Proteins at the Electron Microscope
Sara Gil-Perotin, A. Cebri嫕-Silla, V. Herranz-P廨ez, P. Garc燰-Belda, S. Gil-Garc燰, M. Fil, J.S. Lee, M.V. Nachury, and Jos?Manuel Garc燰-Verdugo
16. Tyramide Signal Amplificaci鏮 for Immunoelectron Microscopy
Mar燰 Jos?Ulloa-Navas, Patricia Garc燰-T嫫raga, Susana Gonz嫮ez-Granero, Pedro P廨ez-Borreda, Vicente Herranz-P廨ez, and Jos?Manuel Garc燰-Verdugo
17. Pre-Embedding Methods for the Localization of Receptors and Ion Channels
Rafael Luj嫕
18. Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels
Rafael Luj嫕 and Masahiko Watanabe
19. High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)
Walter A. Kaufmann, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto
20. Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM
Marta Tur嶲ano-L鏕ez, Jos?Rodrigo-Rodr璲uez, Lidia Alonso-Nanclares, Juncal Gonz嫮ez-Soriano, Javier DeFelipe, and 聲gel Merch嫕-P廨ez
21. Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies
Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta
22. Efficient Labeling of Neurons and Identification of P
