In addition to updating important core techniques this third edition presents new chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and pericyte isolation techniques, spheroi
This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the underst
This book provides a practical and self-contained overview of the Gene Ontology (GO), the leading project to organize biological knowledge on genes and their products across genomic resources. Written
This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vit
In recent years, single nucleotide polymorphisms have received increased and special attention in a rapidly developing field of personalized medicine and drug treatment. Comprising more than eighty pe
In the decade since publication of the first edition this book, the field has seen several major developments. These developments have both accelerated the pace of structure determination and made cry
Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1 is composed
This volume provides a detailed step-by-step description of protocols for the establishment ofin vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria.The B
This volume guides readers through the field of systems medicine by defining the terminology, and describing how established computational methods form bioinformatics and systems biology can be taken
This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for theMethods in Mol
This volume seeks to understand how organisms and gene functions are influenced by environmental cues while accounting for variation that takes place within and among environmental populations and com
In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessib
The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for sta
This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; d
Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many
This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. Th
This volume discusses experimental brain injury models thatcontain valuable information carefully chosen to widen the researchers’ horizonabout neurotrauma. Injury Models of Central Nervous System: Me
This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and
This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described
