Methods

This volume brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autoimmunity: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This volume covers the latest techniques used to study single nucleotide polymorphisms (SNP) genotyping, such as Sanger sequencing, qPCR, Nanopore sequencing, and PACE. The chapters in this book cover topics such as SNP nomenclature, databases, and significance of SNPs in various fields; PCR-based techniques like HRM and CD-PCR; isothermal amplification methodologies; high-throughput methods like FCS-based SNP genotyping; and guidance of data analysis, emphasizing the importance of quality control, statistical analysis, and visualization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Genotyping: Methods and Protocols is a valuable resource for both new and experienced researchers who want to gain a deeper underst

This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. Furthermore, this new edition introduces new approaches for quantifying immune-active proteins and peptides and explores the increasing role of multi-omics strategies to help researchers further understand immune function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunoproteomics: Methods and Protocols, Third Editi

This second edition volume brings together some of the best experts in the field of modern metabolomics to discuss the latest various techniques used to study specific metabolite classes, and metabolomics in bacterial and mammalian systems. The chapters in this book cover topics such as handling big data for metabolite identification and quantification, as well as building pathways for comparison with other omes, Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial metabolites analysis by mass spectrometry; the metabolomic study of tissues in different diseases; and NMR analysis in livestock metabolomics. It also includes several chapters on the emerging area of spatial metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily repro