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Methods

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出版日:2025/12/07 作者:Rami Bechara(EDI)  出版社:Humana Pr  裝訂:精裝
This volume brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Autoimmunity: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
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出版日:2025/11/02 作者:Kelly M. Fulton(EDI)  出版社:Humana Pr  裝訂:精裝
This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. Furthermore, this new edition introduces new approaches for quantifying immune-active proteins and peptides and explores the increasing role of multi-omics strategies to help researchers further understand immune function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunoproteomics: Methods and Protocols, Third Editi
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出版日:2025/07/24 作者:Anthony Torres(EDI)  出版社:Humana Pr  裝訂:精裝
This volume covers the latest techniques used to study single nucleotide polymorphisms (SNP) genotyping, such as Sanger sequencing, qPCR, Nanopore sequencing, and PACE. The chapters in this book cover topics such as SNP nomenclature, databases, and significance of SNPs in various fields; PCR-based techniques like HRM and CD-PCR; isothermal amplification methodologies; high-throughput methods like FCS-based SNP genotyping; and guidance of data analysis, emphasizing the importance of quality control, statistical analysis, and visualization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Genotyping: Methods and Protocols is a valuable resource for both new and experienced researchers who want to gain a deeper underst
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出版日:2025/06/07 作者:Sanjoy K. Bhattacharya(EDI)  出版社:Humana Pr  裝訂:精裝
This second edition volume brings together some of the best experts in the field of modern metabolomics to discuss the latest various techniques used to study specific metabolite classes, and metabolomics in bacterial and mammalian systems. The chapters in this book cover topics such as handling big data for metabolite identification and quantification, as well as building pathways for comparison with other omes, Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial metabolites analysis by mass spectrometry; the metabolomic study of tissues in different diseases; and NMR analysis in livestock metabolomics. It also includes several chapters on the emerging area of spatial metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily repro
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出版日:2026/02/14 作者:Alexander Deiters(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2023/12/14 作者:Massimiliano Valeriani(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2023/07/30 作者:Alexander N. Freiberg(EDI)  出版社:Humana Pr  裝訂:精裝
This volume summarizes key methods and advancements in the field of henipavirus molecular biology. Chapters guide readers through protocols focusing on emerging bat-borne paramyxoviruses as well as pathogenicity caused by Nipah and Hendra viruses. Protocols are also included to help develop diagnostic tools, antiviral therapeutics, and vaccine creation. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Nipah Virus: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
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出版日:2010/11/30 作者:Helio Chiarini-garcia (EDT); Rossana C. N. Melo (EDT)  出版社:Humana Pr  裝訂:精裝
Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics
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出版日:2025/10/19 作者:Kevin Flanigan(EDI)  出版社:Humana Pr  裝訂:精裝
This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related diseas
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出版日:2025/03/26 作者:Ayan Chatterjee(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/03/04 作者:M. Tofazzal Islam(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2023/03/16 作者:Nicholas Paul Holmes(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2023/01/02 作者:Dhanasekaran Dharumadurai(EDI)  出版社:Humana Pr  裝訂:平裝
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出版日:2022/08/07 作者:Jordi Llorens(EDI)  出版社:Humana Pr  裝訂:平裝
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出版日:2021/08/19 作者:Dhanasekaran Dharumadurai(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2021/07/14 作者:M. Tofazzal Islam(EDI)  出版社:Humana Pr  裝訂:平裝
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出版日:2021/07/09 作者:Jordi Llorens(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2024/11/23 作者:Célia Baroux(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2024/06/09 作者:Celeste M. Nelson(EDI)  出版社:Humana Pr  裝訂:精裝
This second edition guides readers through experimental and computational techniques on the study of tissue morphogenesis, with a specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.
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出版日:2023/07/23 作者:José Luis Riechmann(EDI)  出版社:Humana Pr  裝訂:精裝
This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
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出版日:2026/04/20 作者:Irit Sagi(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2026/02/23 作者:Katrin Janik(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/12/09 作者:Stephan Wenkel(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/11/29 作者:Tanya Radoeva(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/11/18 作者:Jan R. Mead(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/11/07 作者:Taiji Kawakatsu(EDI)  出版社:Humana Pr  裝訂:平裝
出版日:2025/10/31 作者:Stephanie Hughes(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/10/15 作者:Andreas Winkler(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/09/28 作者:Franck Pellestor(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/08/12 作者:Emilio Lecona(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/08/05 作者:Prabhakara P. Reddi(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/07/04 作者:Michael Pepper(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/07/04 作者:Manon M. J. Cox(EDI)  出版社:Humana Pr  裝訂:平裝
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出版日:2025/06/29 作者:Carlo Salerno(EDI)  出版社:Humana Pr  裝訂:平裝
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出版日:2025/06/13 作者:Berkley J. Walker(EDI)  出版社:Humana Pr  裝訂:平裝
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出版日:2025/06/04 作者:Joseph Deweese(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/05/12 作者:Claudia Stange Klein(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/05/03 作者:Luc Gaudreau(EDI)  出版社:Humana Pr  裝訂:精裝
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出版日:2025/04/28 作者:Leonid L. Moroz(EDI)  出版社:Humana Pr  裝訂:平裝
出版日:2025/04/23 作者:Sarah Covshoff(EDI)  出版社:Humana Pr  裝訂:平裝
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