Quantification Addendum

This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related diseas

This second edition volume brings together some of the best experts in the field of modern metabolomics to discuss the latest various techniques used to study specific metabolite classes, and metabolomics in bacterial and mammalian systems. The chapters in this book cover topics such as handling big data for metabolite identification and quantification, as well as building pathways for comparison with other omes, Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial metabolites analysis by mass spectrometry; the metabolomic study of tissues in different diseases; and NMR analysis in livestock metabolomics. It also includes several chapters on the emerging area of spatial metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily repro