Since the identification of the first matrix metalloprotease (interstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapter
This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that
This second edition provides updated and expanded chapters covering a broad sampling of useful and current methods in the rapidly developing and expanding field of bioinformatics. Bioinformatics, Volu
This volume provides protocols for RNA structure modeling and determination. Chapters guide the reader through protocols for RNA secondary structure prediction, single sequence modeling, Crumple, RN
The establishment, analysis, prediction, and expansion of a comprehensive ligand-target Structure-Activity Relationship (SAR) in the post-genomic era presents a key research challenge for this century
As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells.
This volume covers several aspects of rational drug design, such as synthesis of novel bioactive drugs; development and application of new methodologies; computational methods valuable for the establi
This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application. Chapters guide the readers through ex
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane p
This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due
This volume covers a large area, from the description of methodologies for data analysis to the real application. Chapters focus on methodologies for preprocessing of microarray data, a survey of miRN
This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs proteins, glycans
Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane
As one of the fastest growing fields of research in the 21st century, nanotechnology is sure to have an enormous impact on many aspects of our lives. Nanostructure Design: Methods and Protocols serve
In this new volume, renowned authors contribute fascinating, cutting-edge insights into microarray data analysis. Information on an array of topics is included in this innovative book including in-dep
This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi
This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi
With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography
Plants have evolved an amazing array of metabolic pathways leading to molecules capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which
The phenomenon of RNA interference has rapidly moved from groundbreaking scientific discovery to promising therapeutic approach. However, even as RNAi-based drugs enter the clinic, significant challe
Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral
The developing of in vivo neuroscience techniques is rapidly improving the specificity and sensitivity of measurements of brain function. However, despite improvements in individual methods, it is be
A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into p
This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided int
This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal mode
This volume contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolom
The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-d
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and
This volumepresents detailed laboratory protocols regarding the three major routetechnologies luciferases, efficient opticalprobes, and applications to visualizing molecular events in living subjects.
Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of met
Helicases are enzymes that utilize the energy derived from NTP hydrolysis to unwind double standed nucleic acids. Significant progress has been achieved in our understanding of the structure and mole
This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and upd
This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, c
This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design
This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and
This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapte
This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beg
This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, develo
