Humana Pr

This volume brings together a collection of methodological chapters for investigating the structure, function, and mechanisms of root microbiome interactions. Beginning with chapters on the chemical and ecological drivers of rhizosphere interactions, the book continues by covering microbial community composition and structure through reliable sampling and sequencing strategies, functional analyses to decipher microbial activity in the rhizosphere, microbial traits and plant-microbe interactions, and practical and applied aspects of rhizosphere research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, The Root Microbiome: Methods and Protocols serves as a comprehensive resource for researchers working on rhizosphere

This volume presents up-to-date methodologies and experimental approaches for studying somatic cells supporting spermatogenesis, called Sertoli cells, across different developmental stages of the male gonad. Chapters guide readers through protocols on applying primarily to the mammalian Sertoli cells, which are the most widely studied, but also covers techniques used in Sertoli cell research in non-mammalian vertebrates. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Sertoli Cells: Methods and Protocols aims to provide a comprehensive collection of laboratory protocols is intended to assist researchers seeking to understand the diverse and complex biology of Sertoli cells and their fundamental role in testicular de