Regulation of Gene Expression by Small RNAs

New Findings Revolutionize Concepts of Gene Function
Endogenous small RNAs have been found in various organisms, including humans, mice, flies, worms, fungi, and bacteria. Furthermore, it’s been shown that microRNAs acting as cellular rheostats have the ability to modulate gene expression. In higher eukaryotes, microRNAs may regulate as much as 50 percent of gene expression.
Regulation of Gene Expression by Small RNAs brings together the pioneering work of researchers who discuss their work involving a wide variety of small RNA regulatory pathways in organisms ranging from bacteria to humans. In addition to exploring the biogenesis and processing of these regulatory RNAs, they also consider the functional importance of these pathways in host organisms. Assisting current and future researchers, this unique groundbreaking work —


Provides a suite of cutting-edge resources for the study of microRNA ontology and function


Includes a technology guide for those seeking to assay microRNA expression


Explores the mechanisms by which microRNAs regulate gene expression in animal cells, including the regulation of gene expression by RNA-mediated transcriptional gene silencing


Discusses a fast and low-cost approach for reversing genetic influences in mammals


Looks at breakthroughs in the use of microRNA-based therapy for HIV and cancer


This volume captures the essence of the breadth and excitement surrounding the newly discovered regulatory roles of small RNAs. The powerful new approach in the study of gene function described in this text is leading to some remarkable findings that have the potential to revolutionize our understanding of genetic function and the treatment of diseases otherwise considered intractable.

MicroMining:
Computational Approaches to microRNA Discovery, A. Grundhoff




Animal microRNA
Gene Prediction, O. Snøve and P. Sætrom






A Suite of
Resources for the Study of microRNA Ontology and Function, P. Sethupathy, M.
Megraw, and A. G. Hatzigeorgiou


Regulation of
Translation and mRNA Stability by Hfq-Binding Small RNAs in Escherichia
coli, H. Aiba




Mechanisms by
which microRNAs Regulate Gene Expression in Animal Cells, Y. Yu and T. W.
Nilsen




The microRNAs of
Caenorhabditis elegans, M. J. Nolde and F. J. Slack




Isolation and
Characterization of Small RNAs in Caenorhabditis elegans, C. Ushida and Y.
Hokii




MicroRNA Tales
in Fly Development, U. Bhadra, S. K. Singh, S.N.C.V.L. Pushpavalli, P. B.
Hajeri, and M. Pal-Bhadra




RNA Interference
and microRNAs in Zebra Fish, A. S. Flynt, E. J. Thatcher, and J. G. Patton




Biogenesis and
Function of Plant microRNAs, Z. Havelda




Endogenous Small
RNA Pathways in Arabidopsis, M. Agarwal, J. Curaba, and X. Chen




How to Assay
microRNA Expression: A Technology Guide, M. Castoldi, V. Benes, and M. U.
Muckenthaler




Methods to
Quantify microRNA Gene Expression, L. A. Neely




Regulation of
Alternative Splicing by microRNAs, R. K. Gaur




Recent Progress
in Polymerase II-Mediated Intronic microRNA Expression Systems, S.-L. Lin
and S.-Y. Ying




MicroRNA-Based
RNA Polymerase II Expression Vectors for RNA Interference in Mammalian Cells,
A. B. Vojtek, K.-H. Chung, P. D. Patel, and D. L. Turner






Transgenic RNAi:
A Fast and Low-Cost Approach to Reverse Genetics in Mammals, L. Qiu and Z.
Xu


Symphony of
AIDS: A microRNA-Based Therapy, Y. R. Fujii






MicroRNAs and
Cancer: Connecting the Dots, S. D. Jayasena


Mammalian
Transcriptional Gene Silencing by Small RNAs, D. H. Kim and J. J. Rossi




Regulation of
Gene Expression by RNA-Mediated Transcriptional Gene Silencing, K. V. Morris