CHEMICAL BIOLOGY - A PRACTICAL COURSE

Take your first steps in combinatorial synthesis or synthesize bioactive molecules such as antibiotics. Search for mutations in DNA using chemical probes or perform a proteome analysis in yeast.
The present selection of 12 inspiring experiments is tailored for maximum learning effect at minimal expense of time and equipment. Almost all currently used laboratory techniques in synthesis and analysis of bioactive compounds are represented at least once.
Abundant practical hints are complemented by a thorough treatment of the underlying theory and mechanisms.

Herbert Waldmann (b 1957) received his PhD in 1985 from the University of Mainz (Germany) with H. Kunz. After postdoctoral studies (1985-1986, Harvard University, George Whitesides) and habilitation (1991, University of Mainz) he was appointed associate professor at the University of Bonn in 1991. In 1993 he moved to the University of Karlsruhe as Full Professor of Organic Chemistry. In 1999 he was appointed Director at the Max Planck Institute of Molecular Physiology in Dortmund, where he is heading the Department of Chemical Biology. He also holds a Professorship in Biochemistry at the University of Dortmund. Herbert Waldmann has been the recipient of the Friedrich Weygand Award for the advancement of peptide chemistry, of the Carl Duisberg Award of the Gesellschaft Deutscher Chemiker, the Otto-Bayer-Award and the Steinhofer Award of the Steinhofer Foundation.
His current research interests include bioorganic chemistry and natural product synthesis as well as biocatalysis, stereoselective synthesis and combinatorial chemistry.

Petra Janning (b. 1967) studied chemistry at the University of M?nster (Germany). She completed her PhD degree at the University of Dortmund with G. T?lg and M. Linscheid. From 1995 - 1998 she worked as a chemist in the University Hospital in Aachen. From 1999-2001 she worked in the group of G.H. Degen and H.M. Bolt at the Institute of Occupational Health in Dortmund. In 2001 she joined the Max Planck Institute of Molecular Physiology, where she is currently working in the group of Herbert Waldmann. She is responsible for the Bioorganic Practical Course and the analytics in the department.

Preface.

Abbreviations.

1. Introduction: Chemical Biology– A New Science at the Crossroads of Chemical and Biology (R. Breinbaur & H. Waldmann).

2. DNA-Synthesis and DNA-Hybridization (S. Ficht, et al.). 

3. Doubly Labeled Peptide Nucleic Acids (PNAs) as Probes for the Detection of DNA Point Mutations (O. Köhler, et al.). 

4. Synthesis and Characterization of a Covalent Oligonucleotide-Streptavidin Conjugate and its Application in DNA-directed Immobilization (DDI) (F. Kukolka, et al.). 

5. Solid Phase Synthesis of Peptides: Bradykinin Analogs and the Evaluation of Calcium Mobilization in PC-12 Cells (C. Katzka, et al.). 

6. In silico Protein Ligand Design (M. A. Koch, et al.). 

7. Lipidation of Proteins and Peptide: Farnesylation of the Ras Protein (S. Sommer, et al.). 

8. Insertion of Lipidated Peptides into Model Membranes (D. Gottieb & A. Watzke).

9. Isolation of Potato Phosphorylase and Enzymatic Synthesis of Amylose (M. Scheck, et al.). 

10. Proteome Analysis: Identification of Proteins Isolated from Yeast (H. Prinz, et al.). 

11. Lectins: Determination of the Sugar Specificity of Jacalin by a Sugar-Lectin Binding Assay (SLBA) (E. Gourzoulidou & S. Schlummer).

12. Combinatorial Synthesis and Genetic Algorithm (T. Lessmann, et al.). 

13. Solid Phase Synthesis of an Antibiotic (C. Peters, et al.). 

Appendix.

Subject Index.