Hyphenated And Alternative Methods of Detection in Chromatography

Widely employed for separating and detecting chemicals in solution, separation techniques are most often applied in tandem, subsequently referred to as hyphenated methods. Hyphenated and Alternative Methods of Detection in Chromatography details the development and application of mass spectral detection techniques coupled with gas phase and liquid phase chromatographies. With contributions from experts in a variety of fields, as evidenced by the range of topics, the book describes the advantages and disadvantages of various separation techniques and addresses methods for cutting-edge applications such as proteomics research.

Specifically, this book covers:


The development of the LC-NMR and its application in chromatographic science, particularly in relation to the analysis of natural and environmental samples and in the study of reaction monitoring, biosynthetic analysis, and structural elucidation
The application of LC-ICP in biological samples for the speciation of organoarsenic and organoselenium compounds; metal complexes in microorganisms, plants, and foods of plant origin; human body fluids and tissues; and in the detection of chemical warfare agents
Electrochemical detection in liquid chromatography, system optimization, and applications in the analysis of flavonoids
The use of chemiluminescence for microcolumn and capillary methods of separation
The process of employing multidimensional separation methods as a means of identifying components in complex samples


A detailed reference for those starting out in the field, this book also suits those who are more experienced yet require assistance in new directions. It is an ideal reference for senior undergraduate and postgraduate students wishing to supplement their learning experience. Containing over 800 references, this book is an excellent source of information within the field of hyphenated methods of chromatographic analysis.

Dr. R. Andrew Shalliker received his Ph.D. in 1992 and his BSc (Hons) in 1987 from Deakin University, Geelong, Australia. He joined the University of Western Sydney (UWS) in 1999, and is currently an Associate Professor of Analytical Chemistry. Prior to joining UWS, he was a research fellow in Professor Guiochon’s group at the University of Tennessee, Knoxville,, and Oak Ridge National Laboratories, Oak Ridge, Tennessee (1997–1999). From 1995 to 1997, he held a Queensland University of Technology (QUT) Postdoctoral Research Award at QUT Brisbane. In 1994, he was an Associate Lecturer of Analytical Chemistry at the University of Tasmania, Hobart. Between 1992 and 1994, he was a research chemist at Faulding-David Bull Laboratories, Melbourne, Victoria, Australia.
Dr. Shalliker’s research interests are in the field of liquid chromatography. He has published over 100 scientific papers in this field, focusing on the areas of column technology, stationary phase design, multidimensional HPLC, polymer and oligomers separations, and the analysis of natural products employing chromatographic separations. In 2007, his research team joined the research groups at UTAS and RMIT as a member of the Australian Centre for Research on Separation Science (ACROSS) consortium.

Mass Spectrometry and Separation ScienceXavier ConlanAnalytical Aspects of Modern Gas Chromatography: Mass SpectrometryShin Miin Song and Philip MarriottCoupling Liquid Chromatography and Other Separation Techniques to Nuclear Magnetic Resonance SpectroscopyCristina Daolio and Bernd SchneiderApplication of Infrared and Raman Spectroscopy for Detection in Liquid Chromatographic SeparationsPeter J. MahonEvaporative Light Scattering and Charged Aerosol DetectorPierre ChaminadeDetection and Determination of Heteroatom-Containing Molecules by HPLC: Inductively Coupled Plasma Mass SpectrometrySandra Mounicou, Kasia Bierla, and Joanna SzpunarHPLC with Electrochemical DetectionFumiyo Kusu and Akira KotaniLiquid-Phase Chemiluminescence Detection for HPLCPaul S. Francis and Jacqui L. AdcockMultidimensional High-Performance Liquid ChromatographyColeen S. Milroy, Paul G. Stevenson, Mariam Mnatasakyan, and R. Andrew ShallikerIndex