Opioid Receptors: Methods and Protocols

Part I: transcriptional and post-transcriptional analysis and cellular detection of opioid receptors

1. Overview of Genetic Analysis of Human Opioid Receptors

Santi M. Spampinato

2. Renilla luciferase Reporter Assay to Study 3'UTR-driven post-Transcriptional Regulation of OPRM1

Gabriele Vincelli and Andrea Bedini

3. Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1

Vittoria Borgonetti and Nicoletta Galeotti

4. Monitoring Opioid Receptors Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)

Monica Baiula

5. Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-visual Arrestins

Andrea Bedini

6. Functionalization and Bioconjugation of Nanoruby for Long-term, Ultrasensitive Imaging of Μ-Opioid Receptors

Rashmi Pillai, Mark Connor, and Varun Sreenivasan

7. Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues

Yvonne Schmidt and Halina Machelska

Melih 畤g Celik, Dominika Labuz, and Halina Machelska

Part II: Analysis of signaling events modulated by opioid receptors

9. Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-tolerant Mice

Vittoria Borgonetti and Nicoletta Galeotti

10. GTPgS-autoradiography for Studies of Opioid Receptor Functionality

Alfhild Gr霵bladh and Mathias Hallberg

11. Evaluating Opioid Mediated Adenylyl Cyclase Inhibition in Live Cells using a BRET-based Assay

Preeti Manandhar, Shivani Sachdev and Marina Santiago

12. Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury

Viola Seitz, Philip St飆zner, Dominika Labuz, and Halina Machelska

13. Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV

Bradley Nash, Elena Irollo, Renato Brandimarti, and Olimpia Meucci

14. An In Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion

Monica Baiula

15. Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat

Alfhild Gr霵bladh, Erik Nylander, Sofia Zelleroth, and Mathias Hallberg

16. The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls and Future Perspectives of Biased Agonists at Opioid Receptors

Andrea Bedini

17. Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment

Gabriele Campana and Roberto Rimondini

18. Evaluation of Murine Macrophage Cytokine Production after In Vivo Morphine Treatment

Silvia Franchi, Mara Castelli, Sarah Moretti, Alberto Panerai, and Paola Sacerdote

19. Measurement of Macrophage Toll like Receptor 4 Expression After Morphine
Treatment

Mara Castelli, Alberto Panerai, Paola Sacerdote, and Silvia Franchi

20. Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test

Maria Scherma, Liana Fattore, Walter Fratta, and Paola Fadda

21. Analysis of Opioid-Seeking Behavior through the Intrave