Part I: Imaging with Passive Optical Readouts
1. Fluorescent Labeling of the Nuclear Envelope without Relying on Inner Nuclear Membrane Proteins
Toshiyuki Taniyama and Shinji Sueda
2. Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools
Osamu Takenouchi, Hideaki Yoshimura, and Takeaki Ozawa
3. Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging
Bryce T. Bajar and Michael Z. Lin
4. A Murine Bone Metastasis Model Using Caudal Artery Injection and Bioluminescence Imaging
Takahiro Kuchimaru and Shinae Kizaka-Kondoh
5. A New Lineage of Artificial Luciferases for Mammalian Cell Imaging
Sung-Bae Kim and Rika Fujii
6. Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems
Jittima Phonbuppha, Ruchanok Tinikul, and Pimchai Chaiyen
Part II: Imaging with Activatable Bioluminescent Probes
7. Quantitative Determination and Imaging of Gαq Signaling in Live Cells via Split-Luciferase Complementation
Timo Littmann, Takeaki Ozawa, and Gther Bernhardt
8. A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules
Qiaojing Li, Hideaki Yoshimura, and Takeaki Ozawa
9. Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semi-Rational Molecular Design and Molecular Evolution
Marie Mita, Devina Wongso, Hiroshi Ueda, Takashi Tsuboi, and Tetsuya Kitaguchi
Part III: Imaging with Functional Substrates and Luciferases
10. Near Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette
Masahiro Abe, Ryo Nishihara, Sung Bae Kim, and Koji Suzuki
11. Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells
Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Koji Suzuki, and Sung-Bae Kim
12. Luciferase-Specific Coelenterazine Analogues for Optical Crosstalk-Free Bioassays
Ryo Nishihara, Masahiro Abe, Koji Suzuki, and Sung-Bae Kim
Part IV: Imaging with Organic Fluorescent Probes
13. Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe
Tadanobu Takahashi, Yuuki Kurebayashi, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, and Takashi Suzuki
14. Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe
Mitsuyasu Kawaguchi and Hidehiko Nakagawa
15. Live-Cell Fluorescence Imaging of Microtubules by Using a Tau-Deriv
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