Single-Cell Protein Analysis: Methods and Protocols
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1. Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections
Preethi Vijayaraj
2. Indirect Immunofluorescence of Tissue Sections
Cody J. Aros
3. Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique
Valentina Proserpio, Laura Conti, and Salvatore Oliviero
4. High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry
Marco A. Fernandez, Hammad Alzayat, Maria C. Jaimes, Yacine Kharraz, Gerard Requena, and Pedro Mendez
5. Detection of Cytokine Secreting Cells by Enzyme-Linked Immunospot (ELISpot)
Bernt Axelsson
6. Detection and Enumeration of Cytokine Secreting Cells by FluoroSpot
Bernt Axelsson
7. Single Cell Protein Profiling by Microdroplet Barcoding and Next Generation Sequencing
Samuel C. Kim, John R. Haliburton, Zev J. Gartner, and Adam R. Abate
8. Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis
翳os V嶲v嫫i, Jimmy E. Rodrigues, and Roman A. Zubarev
9. Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels
Carlene Petes, Stephen K.H. Li, Shariq Mujib, Michelle M. Poulin, Noah Saederup, Andrew A. Quong, and Christina Loh
10. High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging
Ofer Elhanani, Leeat Keren, and Michael Angelo
11. Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA
Jing Lin and Savaş Tay
12. High-Throughput Multimodal Single-Cell Targeted DNA and Surface Protein Analysis Using the Mission Bio Tapestri Platform
David W. Ruff, Dalia Dhingra, Kathryn Thompson, Jacqueline A. Marin, and Aik T. Ooi
13. Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform
Nicole Mende, Elisa Laurenti, Berthold Gottgens, and Nicola K. Wilson
14. Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequencing Datasets
Benjamin Lacar
15. Simultaneous Quantification of Single-Cell Proteomes and Transcriptomes in Integrated Fluidic Circuits
Mandi Wong, Carol Kosman, Liane Takahashi, and Naveen Ramalingam
16. Combined Measurement of RNA and Protein Expression on a Single-Cell Level
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