Axonal Transport: Methods and Protocols

Part I: Squid, Aplysia, and Xenopus

1. The Giant Axon of the Squid: A Simple System for Axonal Transport Studies

Joseph DeGiorgis, Marcus Jang, and Elaine L Bearer

2. Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica

Kerriann Badal, Yibo Zhao, Kyle Miller, and Sathyanarayanan Puthanveettil

3. Live imaging of RNA Transport and Translation in Xenopus Retinal Axons

Julie Qiaojin Lin and Jean-Michel Cioni

Part II: Mouse in vivo and ex vivo

4. Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves

Stacey Anne Gould, Robert Adalbert, Stefan Milde, and Michael Coleman

5. In vivo Imaging of Axonal Organelle Transport in the Mouse Brain

Johannes Knabbe, Jil Protzmann, and Thomas Kuner

6. Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)

Elaine L. Bearer, Xiaowei Zhang, and Russell E. Jacobs

Part III: Rodent Primary Neurons

7. Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins

Ariel Ionescu and Eran Perlson

8. Imaging Diversity in Slow Axonal Transport

Archan Ganguly and Subhojit Roy

9. Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses

Wesley M. Tierney, Ian A. Vicino, Stella Y. Sun, Wah Chiu, Esteban A. Engel, Matthew P. Taylor, and Ian B. Hogue

10. Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation

Silvia Turchetto, Romain Le Bail, Loic Broix, and Laurent Nguyen

11. Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons

Tabitha J. Hees and Angelika B. Harbauer

12. Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons

Andrew Montgomery, Alex Garbouchian, and Marvin Bentley

13. Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons.

Arman Shekari and Margaret Fahnestock

14. Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons

Maria Fransiska Emily, Lokesh Agrawal, Paolo Barzaghi, Miki Otsuki, and Marco Terenzio

Part IV: Human-derived Neurons

15. High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells

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