The Inflammasome
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1. Assessment of ASC Oligomerization by Flow Cytometry
Laura Hurtado-Navarro, Alberto Baroja-Mazo, Helios Mart璯ez-Banaclocha, and Pablo Pelegr璯
2. In-vitro Assays to Study Inflammasome Activation in Primary Macrophages
Ishita Banerjee
3. Measuring Non-Canonical Inflammasome Activation In Neutrophils
Mercedes Monteleone and Dave Boucher
4. Gasdermin D Cleavage Assay Following Inflammasome Activation
Louisa Janice Kamajaya and Dave Boucher
5. Activation of the Non-Canonical Inflammasome In Mouse And Human Cells
Jelena S. Bezbradica, Rebecca C. Coll, and Dave Boucher
6. Measurement of Inflammasome-Induced Mitochondrial Dysfunction By Flow Cytometry
Mayoorey Murugathasan and Ali A. Abdul-Sater
7. Detection of ASC Oligomerization by Western Blotting
Safoura Zangiabadi, Ali Akram, and Ali A. Abdul-Sater
8. Reconstitution System of NLRP3 Inflammasome in HEK293T Cells
Sheng Chen, Zhexu Chi, and Di Wang
9. Intracellular Potassium Ions Measurements by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES)
Yifei Zhang and Yan Shi
10. NLRP3 Phospho-Residue Mapping by Phospho Dot-Blots
Sangeetha Shankar, Zsofia Bittner, Alexander N. R. Weber
11. Analysis of Activity and Expression of the NLRP3, AIM2 and NLRC4 Inflammasome in Whole Blood
Lev Grinstein and Stefan Winkler
12. Measurement of Cytosolic Mitochondrial DNA after NLRP3 Inflammasome Activation
Olga M. Ant鏮 and Javier Traba
13. Inflammasome-derived Exosomes Isolation and Imaging by Transmission Electron Microscopy
Yuehui Zhang and Jian Wang
14. In Vitro and In Vivo Model for Sepsis through Non-Canonical NLRP3 inflammasome Activation
Xiao Sun, Tae-Bong Kang, and Kwang-Ho Lee
15. Inflammasome Activation in Gingival Epithelial Cells
Kalina R Atanasova and 畤lem Yilmaz
16. Imaging of Inflammasome Speck Formation in Living Cells
Lucas Secchim Ribeiro
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