Microtubules: Methods and Protocols

PART I: Preparation and Modification of Microtubules

1. Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification

Satsuki Ishii, Mousumi Akter, Jakia Jannat Keya, Mst. Rubaya Rashid, Farhana Afroze, Syeda Rubaiya Nasrin, and Akira Kakugo

2. Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule Associated Intracellular Function

Satyajit Ghosh, Shubham Garg, Nabanita Mukherjee, and Surajit Ghosh

3. Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA

Mousumi Akter, Jakia Jannat Keya, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, Satsuki Ishii, and Akira Kakugo

4. Electro-Modulation of Tubulin Properties and Function

Djamel Eddine Chafai and Michal Cifra

PART II: Observation and Control of Microtubule Movement

5. In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference Reflection Microscopy

Yin-wei Kuo and Jonathon Howard

6. Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microscopy

Virginia VanDelinder and George D. Bachand

7. Design of Mechanical and Electrical Properties for Multi-Directional Control of Microtubules

Hang Zhou, Taikopaul Kaneko, Naoto Isozaki, and Ryuji Yokokawa

Kazuya Fujimoto, Ryota Iino, and Ryuji Yokokawa

9. Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bionanodevices

Xiao Hu and Cerasela Zoica Dinu

PART III: Microtubule-Based Active Matters

Alexandra M. Tayar, Linnea M. Lemma, and Zvonimir Dogic

11. Spontaneous Alignment of Microtubules via Tubulin Polymerization in a Narrow Space under a Temperature Gradient

Kazuhiro Shikinaka

12. Dynamic Pattern Formation of Active Matters Triggered by Mechanical Stimuli

13. Spontaneously Beating Biomimetic Structures

Isabella Guido

14. Construction of Molecular Robots from Microtubules for Programmable Swarming

Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, and Akira Kakugo

15. Fabrication of Artificial Muscle from Microtubules, Kinesins and DNA Origami Nanostructures

Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Satsuki Ishi, and Akira Kakugo

PART IV: Microtubule-Binding Molecules

16. Encapsulation of Nanomaterials Inside Microtubules by Using a Tau-derived Peptide

Hiroshi Inaba and Kazunori Matsuura

17. Investigating Tubulin-Drug Interaction Using Fluorescence Spectroscopy

Anuradha Kumari and Dulal Panda

PART V: Analysis of Microtubule Dynamics and Structures

18. Visualization and Quantification of Microtubule Self-repair

J廨幦ie Gaillard, Laurent Blanchoin, Manuel Th廨y, and Laura Schaedel

19. Cargo Transport by Microtubule-Associated Motor Protein Along Mechanically Deformed Microtubules

Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, and Akira Kakugo

20. Mechanical Deformation of Microtubules on a Two-Dimensional Elastic Medium

Syeda Rubaiya Nasrin, Farhana Afroze, Arif Md. Rashedul Kabir, and Akira Kakugo

21. Real-Time Imaging of Single