Preface...
Table of Contents...
Contributing Authors...
Part I ES/iPS
1. Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cells
Xiugong Gao, Robert L. Sprando, and Jeffrey J. Yourick
2. Artificial Activation of Murine Oocytes using Strontium to Derive Haploid and Diploid Parthenotes
Daphne Norma Crasta, Satish Kumar Adiga, Nagarajan Kannan, and Guruprasad Kalthur
3. Generation of Human iPSC from Small Volume Peripheral Blood Samples
Doreen Kloos and Nico Lachmann
4. Distinguishing between Endodermal and Pluripotent Stem Cell Lines during Somatic Cell Reprogramming
A. Moauro and A. Ralston
5. Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells
Alexander Keller, Nusa Krivec, Christina Markouli, and Claudia Spits
6. Detection of Soluble and Insoluble Protein Species in Patient-Derived iPSCs
Stephanie Santarriaga, Ian Luecke, and Allison D. Ebert
7. Extracellular Flux Analysis of Mitochondrial Function in Pluripotent Stem Cells
Enkhtuul Tsogtbaatar, Katherine Minter-Dykhouse, Alicia Saarinen, and Clifford D. L. Folmes
8. Assessment of Endothelial-to-Hematopoietic Transition of Individual Hemogenic Endothelium and Bulk Populations in Defined Conditions
Gene I. Uenishi, Ho Sun Jung, and Igor I. Slukvin
Part II ES/iPS Derived Tissue Stem Cells
9. Homogenous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells
Rong Li, Yang Zhao, Jeffrey J. Yourick, Robert L. Sprando, and Xiugong Gao
10. Differentiation of Human Induced Pluripotent Stem Cells into Cortical Neurons to Advance Precision Medicine
M. Catarina Silva, Ghata Nandi, and Stephen J. Haggarty
11. Differentiation of iPS-Cells into Peripheral Sensory Neurons
Anika Neureiter, Esther Eberhardt, and Angelika Lampert
12. Culture of Human iPSC-Derived Motoneurons in Compartmentalized Microfluidic Devices and Quantitative Assays for Studying Axonal Phenotypes
Maria Giovanna Garone, Chiara D'Antoni, and Alessandro Rosa
13. iPS Cell-Differentiation into Brain Microvascular Endothelial Cells
Angelica Medina and Hengli Tang
14. Chromatin Immunoprecipitation in Human Pluripotent Stem Cell-Derived 3D Organoids to Analyze DNA-Protein Interactions
Wei Xuan Tan, Chek Mei Bok, Natasha Hui Jin Ng, and Adrian Kee Keong Teo
15. Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells
Mengcheng Shen, Chun Liu, and Joseph C. Wu
16. Generation of Salivary Gland Organoids from Mouse Embryonic Stem Cells
Junichi Tanaka and Kenji Mishima
17. In Vitro Generation of Heart Field Specific Cardiomyocytes
Arash Pezhouman, Ngoc B. Nguyen, Allison Shevtsov, Rong Qiao, and Reza Ardehali
Part III Tissue Stem Cells
18. Isolation and Characterization of Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells
Noridzzaida Ridzuan, Darius Widera, and Badrul Hisham Yahaya
19. Identification and Validation of CRISPR/Cas9 Off-Target Activity in Hematopoietic Stem and Progenitor Cells
So Hyun Park, Ciaran M. Lee, and Gang Bao
20. Genome Engineering of Hematopoietic Stem Cells using CRISPR/Cas9 System
Nivedhitha Devaraju, Vignesh Rajendiran, Nithin Sam Ravi, and Kumarasamypet M. Moahnkumar
21. Generation of Rat Neural Stem Cells to Produce Different Astrocyte Phenotypes
Rebecca Sherrard Smith, Susan C. Barnett, and Susan L. Lindsay
22. In Situ Quantification and Isolation of Mler Glial Cells by Fluorescence Activated Cell Sorting from the Regenerating Larval Zebrafish Retina
Jeffrey Stulberg and Vincent Tropepe
23. Quantification and Cloncal Culture of Neural Stem Cells from the Hippocampus of Adult Mouse
Yoko Amagase, Hiroko Izumi-Nakaseko, Atsushi Sugiyama, and Yoshinori Takei
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