Preface...Table of Contents...Contributing Authors...
1. Glycosylation of Therapeutic Proteins: A Critical Quality AttributeArnaud Delobel
2. Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass SpectrometryDan Bach Kristensen, Trine Meiborg Sloth, Martin rgaard, and Pernille Foged Jensen
3. Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass SpectrometryEstela Gim幯ez, Montserrat Mancera-Arteu, Fernando Benavente, and Victoria Sanz-Nebot
4. Fc Glycosylation Characterization of Human Immunoglobulins G using Immunocapture and LC-MSYosra Helali, Shilpee Sharma, Marie Vandeput, Dansala Welba, Pierre Van Antwerpen, Arnaud Marchant, and C嶮ric Delporte
5. Fast Afucosylation Profiling of Glyco-Engineered Antibody Subunits by Middle Up Mass SpectrometryElsa Wagner-Rousset, Olivier Colas, St廧hane Chenu, Yannis-Nicolas Fran蔞is, Davy Guillarme, Sarah Cianferani, Yury O. Tsybin, Jonathan Sj鐷ren, Arnaud Delobel, and Alain Beck
6. Characterization of Glycosylated Proteins at Subunit Level by HILIC/MSValentina D'Atri and Davy Guillarme
7. Analysis of Monoclonal Antibody Glycopeptides by Capillary Electrophoresis - Mass Spectrometry Coupling (CE-MS)Josiane Saad? Michael Biacchi, J廨幦ie Giorgetti, Antony Lechner, Alain Beck, Emmanuelle Leize-Wagner, and Yannis-Nicolas Fran蔞is
8. Enrichment of Intact Glycopeptides using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction ChromatographyAbel Bermudez and Sharon J. Pitteri
9. In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC -SPE and LC-MS/MSMyung Jin Oh, Youngsuk Seo, Unyong Kim, and Hyun Joo An
10. Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSEKevin Canis, Estelle Gar幯aux, and Jean-Fran蔞is Boe
11. Mapping O-glycosylation Sites using OpeRATOR and LC-MSMaria Nordgren, Andreas N輍eli, Hel幯 Nyhl幯, and Jonathan Sj鐷ren
12. Site-Specific O-Glycosylation Analysis by Liquid Chromatography/Mass Spectrometry with Electron-Transfer/Higher-Energy Collisional DissociationNoritaka Hashii and Junya Suzuki
13. Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS after Derivatization with Fluorescent AnthranilamideClaire I. Butr? Eric Largy, and Arnaud Delobel
14. Evaluating N-glycosylation of a Therapeutic Monoclonal Antibody using UHPLC-FLR-MS with RapiFluor-MS LabelingRosie Upton, James Duffy, Sam Clawson, and David Firth
15. Routine Analysis of N-glycans using Liquid Chromatography Coupled to Routine Mass DetectionXimo Zhang, Vithiya Vimalraj, and Mitul Patel
16. Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals Sonja Schneider, Edgar Naegele, and Sonja Krieger
17. Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS after Derivatization with Fluorescent AnthranilamideClaire I. Butr? Eric Largy, Fabrice Cantais, and Arnaud Delobel
18. Linkage Analysis of Oligo- and Polysaccharides: A TutorialIan Black, Christian Heiss, Russell W. Carlson, and Parastoo Azadi
19. Use of Exoglycosidases for the Structural Characterization of GlycansBeth McLeod, Paula Magnelli, and Xiaofeng Shi
20. Determination of Isomeric Glycan