Part I: The Spectrum of Mouse Primed Pluripotent Stem Cells
1. Establishment of Mouse Epiblast Stem Cells
Pierre Osteil
2. L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell
Hannah J. Glover, Rachel A. Shparberg, and Michael B. Morris
3. Generation of Epiblast-Like Cells
Federica Cermola, Eduardo J. Patriarca, and Gabriella Minchiotti
Part II: Toolkits to Characterize and Study Primed Stem Cells
4. Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence
V. Pragathi Masamsetti and Patrick P.L. Tam
5. Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells
Georgia R. Kafer
6. Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution
Carolyn Sangokoya
7. 3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells
Joshua G. Dierolf, Andrew J. Watson, and Dean H. Betts
8. Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Na鴳e, Formative, and Primed Pluripotent Stem Cells
Joshua G. Dierolf, Kristin Chadwick, Courtney R. Brooks, Andrew J. Watson, and Dean H. Betts
9. Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq
Nazmus Salehin, Nicole Santucci, Pierre Osteil, and Patrick P.L. Tam
10. A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data
Nader Aryamanesh
11. Complete Transcriptome Analysis by 5'-End Single-Cell RNA-Seq with Random Priming
Tsukasa Kouno, Piero Carninci, and Jay W. Shin
12. Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification
Aseel Sharaireh, Anna L. Tierney, and Richard D. Unwin
13. Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-Like Cells
Federico Pecori, Hisatoshi Hanamatsu, Jun-ichi Furukawa, and Shoko Nishihara
Part III: Primed Stem Cells to Engineer Gastrulation Model
14. Generation of Gastruloids from Epiblast-Like Cells
Federica Cermola, Eduardo J. Patriarca, and Gabriella Minchiotti
15. Definitive Endoderm from EpiSC Aggregates in Matrigel
Hisato Kondoh and Mai Fujii
16. In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-Like Cells (PGCLCs)
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