Part I: Cardiac Injury Models
1. Myocardial Infarction Techniques in Adult Mice
Elad Bassat, Dahlia Perez, and Eldad Tzahor
2. Apical Resection and Cryoinjury in Neonatal Mouse Heart
Hua Shen, Ali Darehzereshki, Henry M. Sucov, and Ching-Ling Lien
3. Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice
Tilman Ziegler, Karl-Ludwig Laugwitz, and Christian Kupatt
4. Myocardial Infarction in Pigs
Andrea B鄣r, Nadja Hornaschewitz, and Christian Kupatt
5. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish
Ines J. Marques, Andr廥 Sanz-Morej鏮, and Nadia Mercader
6. Cardiac Resection Injury in Zebrafish
Delicia Z. Sheng, Dawei Zheng, and Kazu Kikuchi
7. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish
Fei Sun, Adam R. Shoffner, and Kenneth D. Poss
8. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration
Yijen L. Wu
Part II: Ex Vivo and In Vivo Approaches
9. Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes
Marina Leone and Felix B. Engel
10. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes
Lu Han, Jocelyn Mich-Basso, and Bernhard Kn
11. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells
Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, and Ravi Karra
12. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells
Jun Xu, Li Wang, Jiandong Liu, and Li Qian
13. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs
Abbigail Helfer and Nenad Bursac
14. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells
Ling Gao and Jianyi Zhang
15. Isolation[SL1] [DC2] and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts
Honghai Liu, Kevin Bersell, and Bernhard Kn
16. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish
Sierra Duca and Jingli Cao
17. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters
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