Part I: Clinical and Diagnostic Virology
1. Evolution and Epidemiology of SARS-CoV-2 Virus
Yu-Nong Gong, Kuo-Ming Lee, and Shin-Ru Shih
2. Molecular Epidemiology of SARS-CoV-2 by Sequencing
Yan Yan and Qinxue Hu
3. Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2
Xuemei Yang, Ning Dong, and Sheng Chen
4. Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance
Vidya Keshav, Lesley Scott, Anura David, Lara Noble, Elizabeth Mayne, and Wendy Stevens
5. Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens
Yasuo Suda, Yasuhisa Tajima, Jun-ichiro Nishi, and Takashi Kajiya
6. Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay
Alexander Wilhelm, Christiane Pallas, Rolf Marschalek, and Marek Widera
7. Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans
Linda Hueston
Part II: Cell Culture, Virus Replication, and Cellular Responses
8. Propagation and Quantification of SARS-CoV-2
Alexander S. Jureka and Christopher F. Basler
9. Quantification of Infectious SARS‐CoV‐2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay
C. Korin Bullen, Stephanie L. Davis, and Monika M. Looney
10. One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Raphael Nyaruaba, Xiohong Li, Caroline Mwaliko, Faith Ogolla, Changchang Li, Lu Zhao, Hang Yang, Junping Yu, and Honping Wei
11. Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics
Lucia Grenga, Duarte Gouveia, and Jean Armengaud
12. Detection of SARS-CoV-2 by Mass Spectrometry
Petra Wandernoth, Katharina Kriegsmann, J顤g Kriegsmann, and Mark Kriegsmann
13. Bioinformatics-Based Approaches to Study Virus-Host Interactions During SARS-CoV-2 Infection
Muhammad Saad Khan, QudsiaYousafi, Shabana Bibi, Muhammad Azhar, and Awais Ihsan
14. Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2
Kai Sen Tan, Akshamal M. Gamage, and Jing Liu
Part III: Immunology and Animal Models
15. Animal Models of COVID-19: Nonhuman Primates
Dhiraj Kumar Singh, Journey Cole, Ruby Escobedo, Kendra J. Alfson, Bindu Singh, Tae-
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