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Cell Cycle Oscillators: Methods and Protocols
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Cell Cycle Oscillators: Methods and Protocols

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1. Cell Cycle Control: A System of Interlinking Oscillators

Randy Y.C. Poon

2. Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity

Matthew P. Swaffer

3. Real Time Monitoring of APC/C-Mediated Substrate Degradation Using Xenopus laevis Egg Extracts

Julia Kamenz, Renping Qiao, Qiong Yang, and James E. Ferrell, Jr.

4. Fluorescent Peptide Biosensors for Probing CDK Kinase Activity in Cell Extracts

Morgan Pellerano and May C. Morris

5. Phosphatase and Kinase Substrate Specificity Profiling with Pooled Synthetic Peptides and Mass Spectrometry

Andrew G. DeMarco, Pete E. Pascuzzi, W. Andy Tao, and Mark C. Hall

6. Whole-Mount Immunostaining for the Identification of Histone Modifications in the S-Phase Nuclei of Arabidopsis Roots

Hirotomo Takatsuka and Masaaki Umeda

7. Construction of a Full-Length 3'UTR Reporter System for Identification of Cell-Cycle Regulating MicroRNAs

Dominika Kazmierczak and Per Hydbring

8. Purification of Cyclin-Dependent Kinase Fusion Complexes for In Vitro Analysis

Mardo K鬑vom輍i

9. Optimizing Cell Synchronization Using Nocodazole or Double Thymidine Block

Arif A. Surani, Sergio L. Colombo, George Barlow, Gemma A. Foulds, and Cristina Montiel-Duarte

10. Highly Synchronous Mitotic Progression in Schizosaccharomyces pombe Upon Relief of Transient Cdc2-asM17 Inhibition

Pawan Singh, Lenka Halova, and Iain Michael Hagan

11. Elucidating Human Mitosis Using an Anaphase-Like Cell-Free System

Danit Wasserman, Sapir Nachum, Meirav Noach-Hirsh, Naomi Auerbach, Evelin Sheinberger-Chorni, Taylor P Enrico, Roxane Lahmi, Michael J. Emanuele, and Amit Tzur

12. EDU (5-Ethynyl-2′-Deoxyuridine)-Coupled-Fluorescence-Intensity Analysis: Determining Absolute Parameters of the Cell Cycle

Jo緌 A. Ferreira, Marco Neves, Miguel Alpalh緌, Pedro Pereira, Daniela Cunha, Fernando Ferreira, Ren?Santus, Ana E. Sousa, and Paulo L. Filipe

13. High-Resolution Analysis of Centrosome Behavior During Mitosis

Vanessa Nunes, Margarida Dantas, Joana T. Lima, and Jorge G. Ferreira

14. Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells

Jonathan M. Geisinger and Tim Stearns

15. Use of Mitotic Protein Kinase Inhibitors and Phospho-Specific Antibodies to Monitor Protein Phosphorylation During the Cell Cycle

Isha Nasa, Greg B. Moorhead, and Arminja N. Kettenbach

16. Visualization of Radiation-Induced Cell Cyc

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定價:100 9279
若需訂購本書,請電洽客服 02-25006600[分機130、131]。