Preface...
Table of Contents...
Contributing Authors...
Part 1 Engineering Mammalian Cells to Sense Biologically Relevant Inputs
1. Generation of CAR-T Cells by Lentiviral Transduction
Atsushi Okuma
2. Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cells
Leo Scheller
3. Engineering Mammalian Cells to Control Glucose Homeostasis
Jiawei Shao, Xinyuan Qiu, and Mingqi Xie
4. Using Engineered Mammalian Cells for an Epitope-Directed Antibody Affinity Maturation System
Akihiro Eguchi and Masahiro Kawahara
5. Purification of Specific Cell Populations Differentiated from Stem Cells using MicroRNA-Responsive Synthetic Messenger RNAs
Hideyuki Nakanishi and Hirohide Saito
Part 2 Engineering Mammalian Cells to Sense Artificial Inputs
6. Green Light-Controlled Gene Switch for Mammalian and Plant Cells
Nils Schneider, Claire V. Chatelle, Rocio Ochoa-Fernandez, Matias D. Zurbriggen, and Wilfried Weber
7. Sonogenetic Modulation of Cellular Activities in Mammalian Cells
Yao-Shen Huang, Ching-Hsiang Fan, Wei-Ting Yang, Chih-Kuang Yeh, and Yu-Chun Lin
8. Constructing Smartphone-Controlled Optogenetic Switches in Mammalian Cells
Yuanhuan Yu, Guiling Yu, and Haifeng Ye
9. Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice
Guiling Yu, Yuanhuan Yu, and Haifeng Ye
10．Construction of Caffeine-Inducible Gene Switches in Mammalian Cells
Daniel Bojar
Part 3 Precise Genome Engineering Techniques using CRISPR-Cas Systems
11. Multiplexed Genome Engineering with Cas12a
Niels R. Weisbach, Ab Meijs, and Randall J. Platt
12. Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells
Soh Ishiguro and Nozomu Yachie
13. Optical Control of Genome Editing by Photoactivatable Cas9
Takahiro Otabe, Yuta Nihongaki, and Moritoshi Sato
Part 4 Engineering Mammalian Cells in Combination with Chemical Compounds/Systems
14. Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT
Sachio Suzuki, Yuka Hatano, Tatsuyuki Yoshii, and Shinya Tsukiji
15． Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules
Hideki Nakamura
16．AgDD System: A Chemical Controllable Protein Aggregates in Cells
Yusuke Miyazaki
17. Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme
Yasunori Okamoto and Ryosuke Kojima
18．Feeder-Free Human Induced Pluripotent Stem Cell Culture using a DNA Aptamer-Based Mimic of Basic Fibroblast Growth Factor
Yuri Hayata, Ryosuke Ueki, and Shinsuke Sando
Part 5 New Techniques to Engineer Specific Mammalian Cells in a Targeted Manner
19. Protocol for de novo Gene Targeting via In Utero Electroporation
Yuji Tsunekawa, Raymond Kunikane Terhune, and Fumio Matsuzaki
20. Magnetically Guided Single Cell Virus Stamping
Rajib Schubert
Subject Index List...
