Part I: The Candida Genome
1. CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans
Ben A. Evans and Douglas A. Bernstein
2. Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species
Lisa Lombardi and Geraldine Butler
3. A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans
Raju Shivarathri, Manju Chauhan, Rounik Mazumdar, Phan Canh Trinh, Wolfgang Reiter, Markus Hartl, Karl Kuchler, and Neeraj Chauhan
4. How to Use the Candida Genome Database
Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock
5. Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata
Eunice L鏕ez-Fuentes, Grecia Hern嫕dez-Hern嫕dez, Alejandro De Las Pe鎙s, and Irene Casta隳
6. Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans
Virginia Glazer and Damian Krysan
Part II: Antifungal Discovery and Resistance
7. Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance
Jane Usher
8. Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS)
Jessica Regan and Glen E. Palmer
9. High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis
Mohit Kumar, Rajendra Prasad, and Ashutosh Singh
10. Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library
Lulu Zhang, Dongmei Li, Richard Calderone, and Xiaodong She
11. Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans
Dongmei Li, Xiaodong She, and Richard Calderone
Part III: Pathogenesis and Virulence
12. In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin
Sejeong Lee, Nessim Kichik, Olivia W. Hepworth, Jonathan P. Richardson, and Julian R. Naglik
13. Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors
Liesbeth Demuyser, Wouter Van Genechten, and Patrick Van Dijck
14. Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy
Junko Yano and Paul L. Fidel, Jr.
15. A Simple Method for Growth of Candida albicans B
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