Part I: Historic Views on the Invention of Lasers and Optical Tweezers
1. The Invention of the Laser
Nick Taylor
2. Art Ashkin and the Origins of Optical Trapping and Particle Manipulation
Matthias D. Koch and Joshua W. Shaevitz
Part II: Technical Advances
3. Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes
Swathi Sudhakar, Viktoria Wedler, Pasupathi Rajendran, and Erik Sch輎fer
4. Angular Optical Trapping to Directly Measure DNA Torsional Mechanics
Xiang Gao, James T. Inman, and Michelle D. Wang
5. Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System
Anna E.C. Meijering, Julia A.M. Bakx, Tianlong Man, Iddo Heller, Gijs J.L. Wuite, and Erwin J.G. Peterman
6. One-Dimensional STED Microscopy in Optical Tweezers
Tianlong Man, Joost J. Geldhof, Erwin J.G. Peterman, Gijs J.L. Wuite, and Iddo Heller
7. Temperature Quantification and Temperature Control in Optical Tweezers
Joost J. Geldhof, Agata M. Malinowska, Gijs J.L. Wuite, Erwin J.G. Peterman, and Iddo Heller
8. High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy
Rajeev Yadav, Kasun B. Senanayake, and Matthew J. Comstock
Part III: DNA, DNA Motors and DNA-Protein Interactions
9. Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers
Graeme A. King, Dian Spakman, Erwin J.G. Peterman, and Gijs J.L Wuite
10. Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay
Arnulf M.K. Taylor, Stephen R. Okoniewski, Lyle Uyetake, and Thomas T. Perkins
11. Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers
Rachel Leicher and Shixin Liu
12. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding
Lulu Bi, Zhenheng Qin, Xi-Miao Hou, Mauro Modesti, and Bo Sun
13. CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy
Matthew D. Newton, Benjamin J. Taylor, Maria Emanuela Cuomo, and David S. Rueda
Part IV: Protein (Un)Folding
14. Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation
Varsha P. Desai, Filipp Frank, and Carlos J. Bustamante
15. Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the H
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