Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols

1. Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory

Y. Victoria Zhang and Uttam Garg

2. System Performance Monitoring in Clinical Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Shannon Haymond

3. Tandem Mass Spectrometry for the Analysis of Plasma/Serum Acylcarnitines for the Diagnosis of Certain Organic Acidurias and Fatty Acid Oxidation Disorders

David Scott, C. Clinton Frazee III, Bryce Heese, and Uttam Garg

4. Quantification of Plasma S-Adenosylmethionine and S-Adenosylhomocysteine Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

Erland Arning, Brandi Wasek, and Teodoro Bottiglieri

5. Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

J. Grace Van Der Gugten and Daniel T. Holmes

6. Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism

Stephen M. Roper, Annette L. Weindel, and Dennis J. Dietzen

7. Quantification of Branched-Chain Amino Acids in Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC- MS/MS)

Hamed Piri-Moghadam, Alan Miller, Debra Pronger, Faye Vicente, Joel Charrow, Shannon Haymond, and David C. Lin

8. Quantitation of Butyrylcarnitine, Isobutyrylcarnitine, and Glutarylcarnitine in Urine Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)

Judith A. Hobert, Stephen A. Brose, and Marzia Pasquali

9. Quantification of Free and Total Carnitine in Serum Using Liquid Chromatography Tandem Mass Spectrometry

Serena Baird, C. Clint Frazee III, and Uttam Garg

10. Sensitive and Robust LC-MS/MS Analysis of Salivary Cortisol in Negative Mode

Wayne B. Anderson, Putuma P. Gqamana, and Y. Victoria Zhang

11. Measurement of Urine Free Cortisol and Cortisone by LC-MS/MS

Julie A. Ray, Erik Kish-Trier, and Lisa M. Johnson

12. Laboratory Diagnosis of Cerebral Creatine Deficiency Syndromes by Determining Creatine and Guanidinoacetate in Plasma and Urine

Ning Liu and Qin Sun

13. Quantitation of Estradiol and Testosterone in Serum Using LC-MS/MS

Ryan C. Schofield, Daniel Kirchoff, and Dean C. Carlow

14. Quantitation of Fatty Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative Chemical Ionization-Mass Spectrometry

Erik Kish-Trier and Tatiana Yuzyuk

15. Quantitation of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry

Erland