1. Need for Methods to Investigate Endocannabinoid Signaling
Mauro Maccarrone
2. Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-like Lipids in Biological Tissues
Claudia Schwitter, Beat Lutz, and Laura Bindila
3. Measuring the Content of Endocannabinoid-like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies
Heather B Bradshaw and Clare T Johnson
4. LC-MS/MS Analysis of AEA and 2-AG
Natalia Battista, Federico Fanti, and Manuel Sergi
5. Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices
Renger F. Witkamp, Ian de Bus, Bauke Albada, and Michiel G.J. Balvers
6. Endocannabinoid-Binding Receptors as Drug TargetsMar燰 G鏔ez-Ca鎙s, Carmen Rodr璲uez-Cueto, Valentina Satta, In廥 Hern嫕dez-Fisac, Elisa Navarro, and Javier Fern嫕dez-Ruiz
7. Assay of CB1 Receptor Binding
Valeria Gasperi, Isabella Savini and Maria Valeria Catani
8. The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
Maria Grazia Cascio, Pietro Marini and Roger G. Pertwee
9. Fluorescence-based Assay for TRPV1 Channels
Aniello Schiano Moriello, Luciano De Petrocellis, Rosa Maria Vitale
10. DNA-Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding
Maria Valeria Catani, Valentina Tullio, Mauro Maccarrone, and Valeria Gasperi
11. Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ and PPARδ Receptors
Uwe Grether, J顤g Benz, Thomas Hartung, and Doris Roth
12. A Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα and PPARγDoris Roth, J顤g Benz, Uwe Grether, and Michel Dietz
13. The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
Pietro Marini, Maria Grazia Cascio, and Roger G. Pertwee
14. Assay of GTPγS Binding in Autoradiography
Marina Gabaglio, Pamela Prini, Erica Zamberletti, Tiziana Rubino, and Daniela Parolaro
15. Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2
Jara Bouma, Marjolein Soethoudt, Noortje van Gils1, Lizi Xia1, Mario van der Stelt2, 3 and Laura H. Heitman
16. Endocannabinoid Metabolism and Transport as Drug Targets
Francesca Ciaramellano, Federico Fanti, Lucia Scipioni, Mauro Maccarrone, and Sergio Oddi
17. Assay of NAT Activity
Toru Uyama and Natsuo Ueda
18. Radiometric Assay of NAPE-PLD activity
Filomena Fezza, Nicolina Mastrangelo, and Mauro Maccarrone
19. A Fluorescence-based NAPE-PLD Activity Assay
Elliot D. Mock, Wouter P. F. Driever, and Mario van der Stelt
20. Radiometric Assay of FAAH Activity
Monica Bari, Monica Feole, Marina Fava, and Mauro Maccarrone
21. Fluorimetric Assay of FAAH activityClotilde B. Angelucci, Roberto Giacominelli-Stuffler, and Mauro Maccarrone
22. Assay of NAAA Activity
Kazuhito Tsuboi and Natsuo Ueda
23. Assay of DAGLα/β Activity
Tiziana Bisogno
24. Assay of Monoacylglycerol Lipase Activity
Kwang-Mook Jung and Daniele Piomelli
25. Radiometric Assay of ABHD2 Activity
Domenico Fazio, Emanuele Criscuolo, and Mauro Maccarrone
26. Oxygenation of Anandamide by Lipoxygenases
Guus van Zadelhoff and Mario van der Stelt
27. Assay of Endocannabinoid Oxidation by Cytochrome P450
Natasha T. Snider and Paul F. Hollenberg
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