This volume discusses the latest advancements in the study of plant transcription factors (TFs), and includes discussions on experimental setups, analytical pipelines, and examples on how to optimize information from databases and resources related to plant TFs. The chapters in this book are organized into three parts. Part One covers high-throughput methodologies for identifying TF binding sites. This part includes standardized and optimized protocols for DAP-seq, ChIP-seq, and CUT&Tag. Part Two centers on the visualization and quantification TF-DNA and TF-protein interactions. This section talks about methodologies used to investigate the localization and timing of transcriptional complex formation in vivo. Part Three explores the integrative frameworks that combine transcriptomics with binding site prediction and gene regulatory networks inference tools. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough,
Plant Transcription Factors: Methods and Protocols is a valuable resource for researchers and laboratories focused on learning more about this important and advancing field.
