The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert research
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an i
Over the last few years, the methods for analyzing cancer related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an i
The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols,
Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investig
Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationall
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols
Since the discovery of DNA structure and throughout the ensuing "DNA era", the field of DNA replication has expanded to cover a vast number of experimental systems. In DNA Replication: Methods and Pr
In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research. In Host-Pathogen Interactions: Methods and Protocols, top experts exami
Exciting work in the past decade has revealed commonalities and differences among individual cell fusion events. In Cell Fusion: Overviews and Methods, a team of leading experts provide a collection
As one of the fastest growing fields of research in the 21st century, nanotechnology is sure to have an enormous impact on many aspects of our lives. Nanostructure Design: Methods and Protocols serve
In this completely revised and updated second edition of DNA Vaccines: Methods and Protocols, W. Mark Saltzman presents a comprehensive collection of DNA vaccine protocols, written by leading experts
A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response a
Provides a sample of applications of biostatistics to commonly occurring analytical problems in molecular biology. The ten papers are arranged in the order in which the methods of analysis would be u
An outstanding collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. The methods span a multidisciplinary range of cellular and molecular appro
This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, c
This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Cha
Ethnobiology and ethnoecology have become very popular in recent years. Particularly in the last 20 years, many manuals of methods have published the most classical approaches to the subject. There ha
This second edition provides detailed LC-MS(/MS) procedures for the analysis of compounds of clinical and toxicological significance. Chapters detail new and updated methods for analyzing drugs focusi
This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and com
This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as
This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and m
This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, bioche
This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental ap
This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their applicatio
This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-
This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchan
This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, th
This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides an
This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA vi
This volume discusses protocols that cover genetic manipulation of Chinese hamster ovary (CHO) cells for recombinant protein production, and protocols for the characterization of CHO cells using ‘omic
This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynami
This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3
Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging tec
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and mo
This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells described are involved in both the growth of the hair follicle and its produc
This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in v
This volume aims to be a collection of essential protocols in iPS cell field.Induced Puripotent Stem Cells (iPS): Methods and Protocols guides readers through multiple facets of stem cell biology, lin
