This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity.
This detailed book provides a comprehensive state-of-the-art presentation of all aspects of miRNA target identification, from the prediction of miRNA binding sites on their target molecules to their e
Conducting Health Research introduces first-year graduate students to the principles and strategies for planning, implementing, reporting, and assessing health research. Comprehensive in its breadth a
This textbook offers a way of gaining the analytic skills essential to undertake intelligence work. It acquaints students and analysts with how intelligence fits into the larger research framework. It
This textbook offers a way of gaining the analytic skills essential to undertake intelligence work. It acquaints students and analysts with how intelligence fits into the larger research framework. It
Intervision is a form of expertise development in which professionals call on their colleagues' peers to help them gain insights into the problems they have at work. A group of five to eight participa
Intervision is a form of expertise development in which professionals call on their colleagues' peers to help them gain insights into the problems they have at work. A group of five to eight participa
This book presents emerging work in the co-evolving fields of design-led systemics, referred to as systemic design to distinguish it from the engineering and hard science epistemologies of system desi
This volume discusses the latest technologies used to study all aspects of Fragile-X Syndrome (FXS). The chapters in this book cover topics such as monitoring for epigenetic modifications at the FMR1
This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that
This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-
This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurologic
This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies t
This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detec
This book presents protocols for identification of genetic drivers of cancer. Chapters guide readers through a brief history of cancer gene discovery, in silico approaches, in vitro&nbs
This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver p
This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research. Split in to three parts, the volume d
This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the vo
This book presents simplified analytical methodologies for static and dynamic problems concerning various elastic thin plates in the bending state and the potential effects of dead loads on static and
This volume details computational techniques for analyses of a wide range of biological contexts, providing an overview of the most up-to-date techniques used in the field. Chapters guide the reader t
This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germ
This book aims to provide scientists with tools and well-researched protocols to enable their research and to facilitate further progress in this common leukemia. Written in the highly successful
This book covers new ways of making materials and compounds in low waste processes employing energy from electricity rather than chemical reagents. Electro organic synthesis offers clean synthesis too
Environmental Economics explores the ways in which economic theory and its applications, as practised and taught today, can be modified to explicitly accommodate the goal of sustainability and the vit
Environmental Economics explores the ways in which economic theory and its applications, as practised and taught today, can be modified to explicitly accommodate the goal of sustainability and the vit
Architectural models are used at various stages of a project. As working models they support the design process: they are made up from time to time using simple materials, such as cardboard, without a
This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such a
This book presents a methodological introduction on clathrin-mediated endocytosis. Chapters guide readers through a number of model systems, purifications, reconstitutions, analysis techniques and sys
This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and b
This text provides a solid foundation in program evaluation, covering the main components of evaluating agencies and their programs, how best to address those components, and the procedures to follow
