This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aime
This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in thi
This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal
This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity,
The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for is
This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book c
This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these tech
This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study. Chapters are divided int
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-
The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionell
This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in
This book aims to promote studies on the entire spectrum of phototropic phenomena in higher and lower plants and fungi. Chapters detail phototropism in many plant species induced by far-red, red, blue
This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and met
This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism. Chapters are divided into three parts, the first part focu
This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kid
This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurologic
G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by le
This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations fo
This book provides a practical guide to current methods for profiling and interpreting genomic alterations in tumors. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenet
This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning
This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome
This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies t
This volume details the most updated concepts and experimental protocols developed by leading researchers in the field. Chapters guide readers through methods on bioinformatics tools, hepatitis c viru
This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to
This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer
This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Cha
This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detec
This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading rese
This book presents protocols for identification of genetic drivers of cancer. Chapters guide readers through a brief history of cancer gene discovery, in silico approaches, in vitro&nbs
This second edition volume expands on the first edition with more detailed methodologies on prenatal testing and diagnosis, and also covers next-generation sequencing techniques. The chapters in this
This detailed book explores techniques commonly used for research into drug repurposing, a well-known strategy to find alternative indications for drugs which have already undergone toxicology and pha
This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver p
This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment
This second edition provides a comprehensive collection of the cutting-edge methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Chapters detai
This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series
This book expands on the previous volumes with new chapters focusing on functional characterization of phage and their proteins, and on the development of phage therapy by outlining novel models. The
This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research. Split in to three parts, the volume d
