This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology,
This volume discusses the latest advancements and technologies used in cancer drug resistance research.Cancer Drug Resistance: Overviews and Methods contains chapters that cover topics such a
This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these path
This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs proteins, glycans
This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number
Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling; Part II describes the image
This volume provides a clear and detailed roadmap of how to design and execute a gene therapy experiment in order to obtain consistent results. Chapters in this book disseminate bits of unknown inform
This volume presents state of the art of methods that can be useful for both basic and translational researchers to conduct chemoprevention preclinical studies. Written in the highly successfulMethods
This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-t
This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to d
This volume explores the still undiscovered secrets of tumor immunology and cancer immunology by discussing the methods and techniques that world-renowned experts in the field use in their laboratorie
This volume provides a detailed step-by-step description of protocols for the establishment ofin vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters,
This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approac
This volume provides up-to-date scientific achievements from the world’s top researchers.Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use
This second edition volume brings together the experiences of leading scientists in the discipline of cancer gene profiling. Because cancer genes can be profiled in many different ways,Cancer Gene Pro
The second edition of this volume focuses on applied bioinformatics with specific applications to crops and model plants.Plant Bioinformatics: Methods and Protocols is aimed at plant biologists who ha
This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for theMethods in Molecular Biology serie
This valuable collection aims to provide a collection of frequently used statistical methods in the field of proteomics. Although there is a large overlap between statistical methods for the different
This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical prop
In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the variou
This volume focuses on malaria vaccinology, and introduces researchers to a complete overview of the protocols and tools used by molecular and cellular malariologists. The chapters within this book ta
This volume details methods and protocols for the synthesis and characterization of glycopolymers and their biomedical applications. Chapters are divided into three parts covering synthesis and charac
This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human
This 3rd edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. ofMitochondrial DNA: Methods
This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell leve
This second volume expands upon the previous edition with new research and objectives in immunoregulation and immune tolerance. Chapters cover topics ranging from new molecular and cellular mechanisms
This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of sp
This volume focuses on screening and profiling kinase inhibitors or activators. Chapters in this book cover several facets of the drug discovery processes starting with target identification, and assa
This volume provides detailed molecular analysis of the associated pathways and cell types involved in Celiac Disease, and embraces new technologies such as next generation sequencing. The book is div
The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models high
This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical as
This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling
This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants inStaphylococcus, as ge
This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or
This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods. Written by a team of internationally renowned
This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general meth
This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level.Post-Transcriptional Gene Regulation, Second Edition is org
This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases.P-Type ATPases: Methods and Protocols provides compr
This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; d