商品簡介
This monograph describes the most common gas chromatographic and infrared spectroscopic official methods required for the determination of trans fatty acids for food labeling purposes. The authors review the current status and limitations of the latest methods and discuss the numerous factors that may have an impact on accuracy and precision. The extensive discussion informs current researchers and analysts in nutrition and food chemistry and technology and should provide a stimulus for improving trans fatty acid methodologies.
作者簡介
Magdi Mossoba and John Kramer
目次
Introduction
Structure and Occurrence of trans Fatty Acids
Industrially Produced trans Fatty Acids
Trans Fatty Acids Derived From Ruminants
Trans Fatty Acids in Humans
Capillary Gas Chromatography
Selection of Fatty Acid Derivative
Selection of a Catalyst for Derivatization of Lipids
Selection of GC Column and Column Length
Limitation of 100 m GC Columns
Differences Between 100 m Cyanopropyl
Polysiloxane GC Columns
Effect of Carrier Gas
Effect of Sample Load
Complementary Silver-Ion Separations of
Geometric Isomers
Resolution of trans Isomers Other Than 18:1
Separation of CLA Isomers Using GC and the
Complementary Ag-HPLC Techniques
GC Standards for FAME Identification
Quantitation of Fatty Acid Methyl Esters by GC
Experience
Official Methods
Ce 1h-05, Ce 1i-07 and Related Methods
Fourier-Transform Infrared Spectroscopy
Instrumentation
Transmission Mode
Attenuated Total Reflection Mode
Trans Fat Infrared Methodology
Transmission FTIR Official Method AOCS
Cd 14-95/AOAC 965.34
ATR-FTIR Official Method AOCS Cd 14d-99/
AOAC 2000.10
Negative Second Derivative ATR-FTIR Methodology
Present Status and Limitations of Official Methods
