Protein Engineering Handbook V 3

This third volume of the Protein Engineering Handbook introduces recent advances in the development of novel, effective, and sustainable catalysts through enzyme engineering pioneered in the four years since the publication of the second volume, in addition to covering some older topics that were not discussed in the previous two volumes. Editors Lutz (chemistry, Emory U., US) and Bornscheuer (biotechnology and enzyme catalysis, U. of Greifswald, Germany) present seventeen papers discussing dirigent effects in biocatalysis, protein engineering guided by natural diversity, protein engineering using eukaryotic expression systems, protein engineering in microdroplets, folding and dynamics of engineered proteins, engineering protein stability, enzymes from thermophilic organisms, enzyme engineering by cofactor redesign, biocatalyst identification by anaerobic high-throughput screening of enzyme libraries and anaerobic microorganisms, organometallic chemistry in protein scaffolds, engineering protease specificity, polymerase engineering from PCR and sequencing to synthetic biology, engineering glycosyltransferases, protein engineering of cytochrome P450 monooxygenases, progress and challenges in computational protein design, simulation of enzymes in organic solvents, and engineering of protein tunnels. Annotation c2013 Book News, Inc., Portland, OR (booknews.com)

Stefan Lutz holds a B. S. degree from the Zurich University of Applied Sciences (Switzerland), and a M.S. degree from the University of Teesside (UK). He then obtained a Ph.D. from the University of Florida and spent three years as a Postdoc with Stephen Benkovic at Pennsylvania State University under a fellowship of the Swiss National Science Foundation. Since 2002 he has been a Chemistry professor at Emory University in Atlanta, Georgia (USA). The research in the Lutz laboratory focuses on the structure-function relationship of proteins through combinatorial protein engineering and design.

Uwe Bornscheuer studied Chemistry at the University of Hannover (Germany), where he obtained a Ph. D. at the Institute of Technical Chemistry. He then spent a postdoctoral year at the University of Nagoya, Japan, before returning to Germany to join the Institute of Technical Biochemistry at the University of Stuttgart. Since 1999 he has been Professor for Biotechnology and Enzyme Catalysis at the University of Greifswald. His main research interest is the application of engineered enzymes in the synthesis of optically active compounds and in lipid modification.