Prins And in Situ PCR Protocols

Primed in situ labeling (PRINS) and in situ polymerase chain reaction (PCR) techniques have become for studying chromosomal aneuploidy and rearrangements, gene localization and expression, and genomic expression. Pellestor (Institute of Human Genetics, Centre National de la Recherche Scientifique, France) presents a completely revised and updated edition of a work describing laboratory procedures in the field, from basic methodology to research and clinical applications. Among the protocols detailed in the 21 chapters are detection and sizing of telomeric and other simple repeats by dideoxy-PRINS, brain tissue preparations for chromosomal PRINS labeling, dual-color PRINS for in situ detection of fetal cells in maternal blood, and application of reverse transcription in situ PCR in cancer analysis. The protocols offer step-by-step laboratory instruction, discussion of the principles behind the technique, lists of equipment and reagents, and tips on troubleshooting and pitfalls. Annotation c2006 Book News, Inc., Portland, OR (booknews.com)