Comprehensive coverage of the entire induced pluripotent stem cell basic work flow
Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.
Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.
Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.
Uma Lakshmipathy, Director R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA
Chad C. MacArthur, Staff Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA
Mahalakshmi Sridharan, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA
Rene H. Quintanilla, Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA
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