商品簡介
Part I: Basic Techniques and Their Applications
1. Physcomitrium patens Protoplasting and Protoplast Transfection
Florence Charlot, Guillaume Goudounet, Fabien Nogu? and Pierre-Fran蔞is Perroud
2. Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici
Shiu-Cheung Lung, Nilanth Yogadasan, Makoto Yanagisawa, and Simon D.X. Chuong
3. Protoplast for Gene Functional Analysis in Arabidopsis
Yanlin Liu and Yan Xiong
4. Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum
Fu-Hui Wu, Yu-Hsuan Yuan, Chen-Tran Hsu, Qiao-Wei Cheng, and Choun-Sea Lin
5. CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration
Raviraj Banakar, Krishan M. Rai, and Feng Zhang
6. Protoplast Isolation and Transfection in Rice
Zhaohui Zhong and Yong Zhang
7. Protoplast Isolation and Transfection in Maize
Monique R. Coy, Shane E. Abbitt, and Mary J. Frank
8. Isolation and Transfection of Maize Endosperm Protoplasts
Yufeng Hu and Yubi Huang
9. Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue
James W. Satterlee and Michael J. Scanlon
10. Protoplast Isolation and Transfection in Wheat
Guangbin Luo, Boshu Li, and Caixia Gao
11. Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses
Zeng-Yu Wang and German Spangenberg
12. Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa)
Minjeong Kang, Keunsub Lee, and Kan Wang
13. Protoplast Isolation, Transfection, and Gene Editing for Soybean (Glycine max)
Gunvant B. Patil, Robert M. Stupar, and Feng Zhang
14. Protoplast Isolation and Transformation in Oil Palm
Mat Yunus Abdul Masani, Ghulam Kadir Ahmad Parveez, Gundula Noll, MD Piji Mohd Al Akmarul Fizree, Ravigadevi Sambanthamurthi, and Dirk Pruefer
Part II: Advanced Systems for High Throughput Analysis and Automation
15. Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells
Ward Decaestecker, Norbert Bollier, Rafael A. Buono, Moritz K. Nowack, and Thomas B. Jacobs
16. Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis via Prot
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