1. Starting a C. elegans Research Laboratory: Practical Advice
Anne C. Hart and Heather. L. Bennett
2. The Basics of Setting Up Successful Teaching Labs and Short-Term Projects with C. elegans
Elizabeth E. Glater
3. Cryopreservation of C. elegans and Other Nematodes with Dimethyl Sulfoxide and Trehalose
Kevin F. O'Connell
4. Genetic Methods for Cellular Manipulation in C. elegans
Menachem Katz
5. Observing and Quantifying Fluorescent Reporters
Sreeparna Pradhan and Michael Hendricks
6. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior
Amelie Bergs, Thilo Henss, Caspar Glock, Jatin Nagpal, and Alexander Gottschalk
7. Optogenetic Perturbation of Individual C. elegans Pharyngeal Neurons while Monitoring Feeding Behavior
Nicholas F. Trojanowski and Christopher Fang-Yen
8. Antibody Staining for Nematodes with Heat-Induced Antigen Retrieval (HIAR)
Curtis M. Loer
9. ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy
Chih-Chieh (Jay) Yu, Danielle M. Orozco Cosio, and Edward S. Boyden
10. A Fusion-PCR Method for Expressing Genetic Tools in C. elegans
Eduard Bokman, Yifat Eliezer, and Alon Zaslaver
11. Approaches for CRISPR/Cas9 Genome Editing in C. elegans
Charlotte J. Martin and John A. Calarco
12. Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans
Sonia El Mouridi and Christian Fr kj r-Jensen
13. Mutation Mapping and Identification by Whole-Genome Sequencing
Harold E. Smith
14. Lipid Extraction and Analysis
Henry H. Harrison and Jennifer L. Watts
15. Isolating Caenorhabditis elegans from the Natural Habitat
Clotilde Gimond, Nausicaa Poullet, and Christian Braendle
16. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans
Ross C. Lagoy, Eric Larsen, Dan Lawler, Hamilton White, and Dirk R. Albrecht
17. Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser
Maria B. Harreguy, Tracy S. Tran, and Gal Haspel
18. An Imaging System for Monitoring C. elegans Behavior and Aging
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