Part I: The Exocytic Pathway
1. Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System
Marine D. Camus and Stephane M. Camus
2. Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo
Julia Dancourt and Gr嶲ory Lavieu
3. Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH)
Cedric S. Asensio
4. Spatial and Temporal Control of Protein Secretion with Light
Ashley M. Bourke and Matthew J. Kennedy
5. Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing
Syed Mukhtar Ahmed
6. Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting
Kunrong Mei, Di-Ao Liu, and Wei Guo
7. Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy
Fei Kang and Herbert Y. Gaisano
8. Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique
Jihong Gong, Xiaofei Yang, and Cong Ma
9. Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons
Tong Wang and Fr嶮廨ic A. Meunier
10. Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse
Tamara Basta, Garry P. Morgan, Eileen T. O'Toole, Nalini R. Rao, Jeffrey N. Savas, and Michael H.B. Stowell
11. In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion
Yinghui Liu, Ruyue He, Min Zhu, and Haijia Yu
12. Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)
Yingke Xu, Luhong Jin, and Derek Toomre
Part II: The Endocytic Pathway
13. Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy
Bijeta Prasai and Justin W. Taraska
14. Endocytosis Assays Using Cleavable Fluorescent Dyes
Shifeng Wang, Chun Wan, Galen T. Squiers, and Jingshi Shen
15. Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2
Bing Wang, Rui Yang, Yuan Tian, and Qian Yin
16. Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches
Amulya Priya and Sunand
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