Analytics of Protein-DNA Interactions

Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has develope

This book covers trends in modern biotechnology. All aspects of this interdisciplinary technology, where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genet

Single Molecule Science (SMS) has emerged from developing, using and combining technologies such as super-resolution microscopy, atomic force microscopy, and optical and magnetic tweezers, alongside sophisticated computational and modelling techniques. This comprehensive, edited volume brings together authoritative overviews of these methods from a biological perspective, and highlights how they can be used to observe and track individual molecules and monitor molecular interactions in living cells. Pioneers in this fast-moving field cover topics such as single molecule optical maps, nanomachines, and protein folding and dynamics. A particular emphasis is also given to mapping DNA molecules for diagnostic purposes, and the study of gene expression. With numerous illustrations, this book reveals how SMS has presented us with a new way of understanding life processes. A must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophy

This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling

Building up from microscopic basics to observed complex functions, this insightful monograph explains and describes how the unique molecular properties of water give rise to its structural and dynamical behaviour which in turn translates into its role in biological and chemical processes. The discussion of the biological functions of water details not only the stabilising effect of water in proteins and DNA, but also the direct role that water molecules themselves play in biochemical processes, such as enzyme kinetics, protein synthesis and drug-DNA interaction. The overview of the behaviour of water in chemical systems discusses hydrophilic, hydrophobic and amphiphilic effects, as well as the interactions of water with micelles, reverse micelles, microemulsions and carbon nanotubes. Supported by extensive experimental and computer simulation data, highlighting many of the recent advances in the study of water in complex systems, this is an ideal resource for anyone studying water at t

Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by prote

The structural biology of protein-nucleic acid interactions is in some ways a mature field and in others in its infancy. High-resolution structures of protein-DNA complexes have been studied since th

The study of the interactions of proteins, whether they be enzymes, regulatory proteins or structural proteins, with nucleic acids is one of the most exciting areas of modern molecular biology. In this 1993 text, Professor Steitz reviews the wide-ranging research in structural studies of DNA-binding proteins and their complexes with DNA. The author clearly and concisely describes the uses of techniques in molecular genetics, DNA synthesis, protein crystallography and nuclear magnetic response. The book, by a now Nobel Prize-winning author, will continue to be enjoyed by anyone with an interest in this exciting area of research.