Genetic Techniques For Biological Research - A Case Study Approach

Molecular Genetic Analysis is an advanced textbook to teach the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology.

This book uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis. It provides enough information so readers will be able to apply the approach to their own research project.

Corinne A. Michels is the author of Genetic Techniques for Biological Research: A Case Study Approach, published by Wiley.

Introduction.

SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM

1. Saccharomyces cerevisiae As a Genetic Model Organism

2. Techniques in Cell and Molecular Biology

3. Saccharomyces Cell Structure

SECTION II: TECHNIQUES OF GENETIC ANALYSIS

4. Mutant Hunts - To select or to screen (perhaps even by brute force)

5. Complementation analysis: How many genes are involved?

6. Epistasis analysis

7. Gene isolation and analysis of multiple mutant alleles

8. Suppression Analysis

9. Enhancement and synthetic phenotypes

10. Two-hybrid analysis

11. Advanced concepts in molecular genetic analysis

12. Genomic Analysis

SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE

Case Study I: Glucose Sensing and Signaling mechanisms in Saccharomyces

Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces

Case Study III: The Cell Division Cycle of Saccharomyces

Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces

Index.