商品簡介
Van der Giezen (School of Biological and Chemical Sciences, Queen Mary, University of London, UK) collects protocols for isolating different organelles and localizing particular proteins, using methods such as light, confocal, and electron microscopy. Unlike the first edition, this second edition places emphasis on protein targeting to cellular compartments in both prokaryotic and eukaryotic systems. For eukaryotic systems, virtually every cellular compartment is treated. Protocols outline techniques for the import of proteins of mitochondria and plastids, the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching, and bacterial protein targeting using the Sec-system, type-V secretion apparatus, and the Tat-pathway. A few chapters describe more generally applicable techniques. Care has been taken to include targeting protocols from different systems, including animal, plant, fungal, and protest models. Annotation c2007 Book News, Inc., Portland, OR (booknews.com)
