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This book provides practical informationabout a whole set of protein experiments for advanced structural biology, suchas x-ray crystallography, NMR, electron microscopy, SAXS, advanced Massspectroscopy, surface plasmon resonance, calorimetry as well as a wide varietyof expression systems including eukaryotic and in vitro expression. Structuralgenomics studies pushed forward the development of automated methods in thefield of structural biology. On the other hand, there are increasing needs forthe structural analysis of difficult targets such as large protein complexesand membrane proteins, which are hard to be accomplished with widely usedautomated methods. Researchers need to know more than standard protocols ofprotein experiments. However, there are few books focusing these difficultproblems. This book gives information to address these difficult problems foradvanced structural biology and helps researchers to develop novel methods.This book will give examples of the development of new methods in proteinexperiments with their theoretical background. This type of information willnot only help the understanding of new methods but also encourage anotherdevelopment of a new technique to solve difficult problems in the proteinexperiments for advanced structural biology. This book is expected to basicallyfit graduate students, postdoctoral researchers, young investigators forunderstanding basic theories for experiments and further practical methods ofadvanced structural biology.
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