1. Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
Alberto Maria Luciano, Rodrigo Garcia Barros, Ana Caroline Silva Soares, Jose Buratini, Valentina Lodde, and Federica Franciosi
2. Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids
Krzysztof Wrzesinski, Helle Sedighi Frandsen, Carlemi Calitz, Chrisna Gouws, Barbara Korzeniowska, and Stephen J. Fey
3. Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation
Kelly C.S. Roballo, Carlos E. Ambrosio, and Juliano C. da Silveira
4. A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors
Malgorzata Duda, Lucia Gizler, and Gabriela Gorczyca
5. Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres
Jeffrey Aalders, Laurens L嶲er, Davide Piras, Jolanda van Hengel, and Sergio Ledda
6. Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models
Antonio Murillo and Marta Mu隳z
7. Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues
Matteo Ghiringhelli, Yousef Abboud, Snizhanna V. Chorna, Irit Huber, Gil Arbel, Amira Gepstein, Georgia Pennarossa, Tiziana A.L. Brevini, and Lior Gepstein
8. Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models
Georgia Pennarossa, Alireza Fazeli, Sergio Ledda, Fulvio Gandolfi, and Tiziana A.L. Brevini
9. Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold
Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, and Tiziana A.L. Brevini
10. A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells
Sharon Arcuri, Fulvio Gandolfi, Edgardo Somigliana, and Tiziana A.L. Brevini
11. 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids
Joel Mario Vej-Nielsen and Adelina Rogowska-Wrzesinska
12. Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics
Helle Sedighi Frandsen, Martina Stampar, Joel Mario Vej-Nielsen, Bojana Zegura, and Adelina Rogowska-Wrzesinska
13. Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC)
Keerthie Dissan
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