Dedication...
Acknowledgement...
Preface...
Table of Contents...
Contributing Authors...
Part I Plant Disease Diagnosis: Traditional Methods
1. Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers
Carla S. Pimentel, Paulo N. Firmino, and Matthew P. Ayres
2. Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests
Ana P廨ez-Sierra, Marilia Horta Jung, and Thomas Jung
3. Field and Laboratory Procedures for Fusarium circinatum Identification and Diagnosis
Cristina Zamora-Ballesteros, Reinaldo Pire, and Julio Javier Diez
Part II DNA Extraction Protocols
4. Rapid Extraction of Plant Nucleic Acids by Microneedle Patch for In-Field Detection of Plant Pathogens
Rajesh Paul, Emily Ostermann, and Qingshan Wei
5. DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues
Domenico Rizzo, Daniele Da Lio, Linda Bartolini, Cristina Francia, Antonio Aronadio, Nicola Luchi, Sara Campigli, Guido Marchi, and Elisabetta Rossi
6. A Rapid Method for Extracting High Quality DNA from Soils
Anna Maria Vettraino and Nicola Luchi
Part III Molecular Methods to Detect Plant Pathogens
7. Invasive Alien Plant Pathogens: The Need of New Detection Methods
Alberto Santini and Duccio Migliorini
8. Detection of Airborne Inoculum of Hymenoscyphus fraxineus: The Causal Agent of Ash Dieback
Miloň Dvoř嫜
9. Molecular Detection of Wheat Blast Pathogen in Seeds
Renaud Ioos
10. Detection and Identification of the Causal Agents of Dothistroma Needle Blight
Funda Oskay, Asko Lehtij酺vi, and Hatice Tuğba Doğmuş Lehtij酺vi
11. The Use of qPCR to Detect Cryphonectria parasitica in Plants
Anne Chandelier
12. Rapid Molecular Diagnostics in the Field and Laboratory to Detect Plant Pathogen DNA in Potential Insect Vectors
Karolina Pusz-Bochenska, Edel P廨ez-L鏕ez, Tim J. Dumonceaux, Chrystel Olivier, and Tyler J. Wist
13. A Panel of Real-Time PCR Assays for the Direct Detection of All the Xylella fastidiosa Subspecies
Jennifer Hodgetts
14. Selective Quantification of Erwinia amylovora Live Cells in Pome Fruit Tree Cankers by Viability Digital PCR
Ricardo Delgado Santander, Katarina Gasic, and Srđan Goran Acimovic
15. Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants
Stefania Tegli, Dario Gaudioso, and Damiano Stefanucci
16. Specific Detection of Pseudomonas savastanoi pathovars: From End-Point PCR to Real-Time PCR and HRMA
Stefania Tegli and Chiara Pastacaldi
Part IV High-Throughput Sequencing
17. Computational Analysis of HTS Data and Its Application in Plant Pathology
Edoardo Piombo and Mukesh Dubey
18. Biomonitoring of Fungal and Oomycete Plant Pathogens by Using Metabarcoding
卌ilie D. Tremblay and Guillaume J. Bilodeau
19. Phytobiome Metabarcoding: A Tool to Help Identify Prokaryotic and Eukaryotic Causal Agents of Undiagnosed Tree Diseases
Carrie J. Fearer, Antonino Malacrin? Cristina Rosa, and Pierluigi Bonello
Part V Plant-Pathogen Interactions
20. Plant-Fungal Interactions: Laser Microdissection as a Tool to Study Cell Specificity
Raffaella Balestrini and Fabiano Sillo
21. Somatic Embryogenesis as a Tool for Studying Grapevine-Virus Interaction
Giorgio Gambino and Irene Perrone
22. A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli
Muhammad Awais Zahid, Ramesh R. Vetukuri, and Erik Andreasson
Part VI Population Genomics in Plant Pathology
23. Genetic Analysis of Plant Pathogens Natural Populations
Fabiano Sillo
24. Microsatellite Genotyping
