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1. Protocol for studying embryonic mammary gland branching morphogenesis ex vivo
Qiang Lan, Jyoti Satta, Satu-Marja Myllym鄢i, Ewelina Trela, Riitta Lindstr闣, Beata Kaczyńska, Johanna Englund, and Marja L. Mikkola
2. Multidimensional fluorescence imaging of embryonic and postnatal mammary gland development
Claudia Caraba鎙 and Bethan Lloyd-Lewis
3. Single-cell transcriptomic and epigenetic analyses of mouse mammary development starting with the embryo
Zhibo Ma, Nikki K. Lytle, Cynthia Ramos, Razia F. Naeem, Geoffrey M. Wahl
4. Computational methods to identify cell fate determinants, identity transcription factors and niche induced signaling pathways for stem cell research Muhammad Ali, Mariana Messias Ribeiro, and Antonio del Sol5. Differential proteomic analysis of complex mixtures by label free nLC MS/MS
Iraide Escob廥, Mikel Azkargorta, Ibon Iloro and Felix Elortza
6. Orthotopic transplantation of mouse mammary epithelial cells
Marisa M. Faraldo, Marina A. Glukhova, and Marie-Ange Deugnier
7. Lineage tracing methods to study mammary epithelial hierarchies in vivo
Ver鏮ica Rodilla and Silvia Fre8. Lentiviral transduction of mammary epithelial cells
Richard Iggo9. Modification of Single Cells within Mouse Mammary Gland Derived Acini via Viral Transduction
Lucia Garcia del Valle, Marta G. Montero, and Martin Jechlinger10. Surgical Procedure for Implantation of Human Tumor Tissue into the Epithelium-free Mammary Fat Pad of Immunocompromised Mice to Generate Patient-Derived Xenografts (PDX)
Lacey E. Dobrolecki and Michael T. Lewis11. The Chicken Embryo Chorioallantoic Membrane (CAM); a Versatile Tool for the Study of Patient-Derived Xenografts
Hugo Villanueva and Andrew G. Sikora12. Intraductal injections into the mouse mammary gland
Erik Oliemuller, Richard Newman, and Beatrice A. Howard13. Modelling breast cancer in organoid and intraductal models
Richard Iggo14. Single organoid droplet-based staining method for high-end 3D imaging of mammary organoids
Jakub Sumbal and Zuzana Koledova
15. Divide and Conquer: Isolating Cell Populations to Investigate how Breast Cancer Risk Factors Alter the Breast Microenvironment
Lisa M. Arendt16. An organotypic assay to study epithelial-fibroblast interactions in human breast
Jakub Sumbal, Thorarinn Gudjonsson, Gunnhildur Asta Traustadottir, and Zuzana Koledova
17. Patient-derived explant cultures of normal and tumour human breast tissue
Isabel Gris-C嫫denas, Miriam R墎ano, and Maria dM. Vivanco18. Negative-Selection Enrichment of Circulating Tumor Cells from Peripheral Blood Using the Microfluidic CTC-iChip
Risa Burr, Jon F. Edd, Brian Chirn, Avanish Mishra, Daniel Haber, Mehmet Toner, and Shyamala Maheswaran
19. Measuring Mechanical Properties of Breast Cancer Cells with Atomic Force Microscopy
Barbara Zbiral, Andreas Weber, and Jos?L. Toca-Herrera
20. Breast cancer stem cells: a clinician s view
Jos?Schneider and Francisco J. Vizoso
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